PMID- 12551745
OWN - NLM
STAT- MEDLINE
DCOM- 20030226
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 72
IP  - 14
DP  - 2003 Feb 21
TI  - The collagenolytic effects of the traditional Chinese medicine preparation, 
      Han-Dan-Gan-Le, contribute to reversal of chemical-induced liver fibrosis in 
      rats.
PG  - 1563-71
AB  - Han-Dan-Gan-Le (HDGL), a Chinese herb preparation composed of Stephaniat 
      tetrandra, Salvia miltorrhiza, Radix paeoniae, Astragalus membranaceus, and 
      Ginkgo biloba, has been used to treat human liver fibrosis. This study was 
      designed to examine the therapeutic effect of HDGL on chemical-induced liver 
      fibrosis in adult Wistar rats. Liver fibrosis was produced in rats by carbon 
      tetrachloride (1.2 ml CCl(4)/kg, 2 times/week, after an initial dose of 5.0 ml 
      CCl(4)/kg, sc), plus a diet of 20% fat, 0.05% cholesterol (continuous) and 30% 
      alcohol in the drinking water ad libitum (every other day) for 8 weeks. HDGL (0.5 
      and 1.0 g/kg, ig, daily for 6 weeks) was administered to rats 72 hrs after the 
      last dose of CCl(4) to examine its therapeutic effects on chemical-induced liver 
      fibrosis. Upon pathological examination, the HDGL treatment had significantly 
      reversed chemical-induced liver fibrosis and other hepatic lesions. Hepatic 
      collagen accumulation induced by CCl(4) was markedly reduced by HDGL treatment, 
      as evidenced by hepatic collagen content and by immunohistochemical analysis of 
      type-I collagen in liver. HDGL appeared to stimulate the collagenolytic process 
      in the liver, as a 30-50% increase in urinary excretion of hydroxyproline was 
      observed with HDGL treatment as compared to rats only given CCl(4). In 
      conclusion, HDGL can effectively reverse chemically induced liver fibrosis, and 
      this appears to be due, at least in part, to the stimulation of hepatic 
      collagenolysis, resulting in a resolution of hepatic fibrosis.
FAU - Li, Chengxiu
AU  - Li C
AD  - Department of Pharmacology, Guiyang Medical College, China. li8@niehs.nih.gov
FAU - Luo, Jun
AU  - Luo J
FAU - Li, Ling
AU  - Li L
FAU - Cheng, Mingliang
AU  - Cheng M
FAU - Huang, Nenghui
AU  - Huang N
FAU - Liu, Jie
AU  - Liu J
FAU - Waalkes, Michael P
AU  - Waalkes MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Collagen Type I)
RN  - 0 (Drugs, Chinese Herbal)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Animals
MH  - Astragalus propinquus
MH  - Carbon Tetrachloride/*toxicity
MH  - Collagen Type I/metabolism
MH  - Drugs, Chinese Herbal/administration & dosage/*pharmacology
MH  - Hydroxyproline/urine
MH  - Injections, Subcutaneous
MH  - Liver/*drug effects/metabolism
MH  - Liver Cirrhosis, Experimental/chemically induced/metabolism/*prevention & control
MH  - Male
MH  - Rats
MH  - Rats, Wistar
EDAT- 2003/01/29 04:00
MHDA- 2003/02/27 04:00
CRDT- 2003/01/29 04:00
PHST- 2003/01/29 04:00 [pubmed]
PHST- 2003/02/27 04:00 [medline]
PHST- 2003/01/29 04:00 [entrez]
AID - S0024320502024487 [pii]
AID - 10.1016/s0024-3205(02)02448-7 [doi]
PST - ppublish
SO  - Life Sci. 2003 Feb 21;72(14):1563-71. doi: 10.1016/s0024-3205(02)02448-7.