PMID- 12553733
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20061115
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 383
IP  - 12
DP  - 2002 Dec
TI  - Expression of hepatocyte growth factor activator inhibitor type 2 (HAI-2) in 
      human testis: identification of a distinct transcription start site for the HAI-2 
      gene in testis.
PG  - 1953-7
AB  - Hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) 
      are recently identified integral membrane Kunitz-type proteinase inhibitors. They 
      have important regulatory roles in pericellular activation of hepatocyte growth 
      factor/scatter factor (HGF/SF) which is critically involved in the development 
      and regeneration of various tissues. Recent reports suggest that HGF/SF is also 
      involved in testicular development and spermatogenesis. In this study, we 
      analyzed the expression of HAIs in the testis. In human testis, HAI-2 was 
      strongly expressed whereas HAI-1 mRNA was hardly detectable. Of interest was the 
      observation that the mRNA size of HAI-2 was shorter in the testis (1.2 kb) than 
      those in the other tissues such as placenta (1.5 kb). Subsequent experiments 
      revealed that there are two major transcription start sites of the HAI-2 gene, 
      which are -30 bp and -360 bp upstream from the translation initiation ATG codon. 
      Although the latter site appeared to be mainly used in the placenta and other 
      non-testicular organs, only the former site is used in testis, resulting in the 
      -300 bp shorter mRNA. An immunohistochemical study using a specific monoclonal 
      antibody raised against human HAI-2 protein indicated that HAI-2 is expressed 
      exclusively in primary spermatocytes. These results suggest a distinct regulation 
      of HAI-2 gene expression in testis and that HAI-2 may play a role in the process 
      of spermatogenesis.
FAU - Yamauchi, Masamichi
AU  - Yamauchi M
AD  - Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, 
      Miyazaki 889-1692, Japan
FAU - Itoh, Hiroshi
AU  - Itoh H
FAU - Naganuma, Seiji
AU  - Naganuma S
FAU - Koono, Masashi
AU  - Koono M
FAU - Hasui, Yoshihiro
AU  - Hasui Y
FAU - Osada, Yukio
AU  - Osada Y
FAU - Kataoka, Hiroaki
AU  - Kataoka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (SPINT2 protein, human)
RN  - 9088-41-9 (Trypsin Inhibitor, Kunitz Soybean)
SB  - IM
MH  - Base Sequence
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Membrane Glycoproteins/*biosynthesis/*genetics
MH  - Molecular Sequence Data
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Testis/*metabolism
MH  - Transcription Initiation Site/*physiology
MH  - *Trypsin Inhibitor, Kunitz Soybean
EDAT- 2003/01/30 04:00
MHDA- 2003/07/12 05:00
CRDT- 2003/01/30 04:00
PHST- 2003/01/30 04:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2003/01/30 04:00 [entrez]
AID - 10.1515/BC.2002.220 [doi]
PST - ppublish
SO  - Biol Chem. 2002 Dec;383(12):1953-7. doi: 10.1515/BC.2002.220.