PMID- 12553782
OWN - NLM
STAT- MEDLINE
DCOM- 20030401
LR  - 20131121
IS  - 0002-7863 (Print)
IS  - 0002-7863 (Linking)
VI  - 125
IP  - 5
DP  - 2003 Feb 5
TI  - Differential receptors create patterns diagnostic for ATP and GTP.
PG  - 1114-5
AB  - Herein we report the combination of a library of resin-bound sensors along with a 
      multicomponent sensor array. This novel combinatorial array sensor system shows 
      selectivity for nucleotide phosphates in solution. The design of the anchored 
      receptor includes a 1,3,5-trisubstituted-2,4,6-triethylbenzene scaffold coupled 
      with peptide libraries. Each chemosensor is placed into a micromachined cavity 
      within a silicon wafer, and the optical changes observed by a charged-coupled 
      device result in near-real-time digital analysis of solutions. A colorimetric 
      displacement assay was performed, and time-dependent imaging studies of the 
      selected sensing ensembles result in a differential responses upon addition of 
      adenosine 5'-triphosphate (ATP), adenosine 5'-monophosphate (AMP), or guanosine 
      5'-triphosphate (GTP). An advantage to this approach is that it creates an array 
      of sensors that gives a fingerprint response for each analyte. Principal 
      component analysis indicates that the library of chemosensors can differentiate 
      between ATP, GTP, and AMP. On the basis of factor loading values, individual 
      sensors from the library were sequenced to elucidate their chemical composition.
FAU - McCleskey, Shawn C
AU  - McCleskey SC
AD  - University of Texas at Austin, Department of Chemistry and Biochemistry, Austin, 
      Texas 78712, USA.
FAU - Griffin, Michael J
AU  - Griffin MJ
FAU - Schneider, Stephen E
AU  - Schneider SE
FAU - McDevitt, John T
AU  - McDevitt JT
FAU - Anslyn, Eric V
AU  - Anslyn EV
LA  - eng
GR  - GM57306/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Cell Surface)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - JU58VJ6Y3B (Guanidine)
SB  - IM
MH  - Adenosine Triphosphate/*analysis
MH  - Biomimetic Materials/chemistry
MH  - Combinatorial Chemistry Techniques/*methods
MH  - Guanidine/*chemistry
MH  - Guanosine Triphosphate/*analysis
MH  - Kinetics
MH  - Oligopeptides/*chemistry
MH  - Receptors, Cell Surface/*chemistry
EDAT- 2003/01/30 04:00
MHDA- 2003/04/02 05:00
CRDT- 2003/01/30 04:00
PHST- 2003/01/30 04:00 [pubmed]
PHST- 2003/04/02 05:00 [medline]
PHST- 2003/01/30 04:00 [entrez]
AID - 10.1021/ja021230b [doi]
PST - ppublish
SO  - J Am Chem Soc. 2003 Feb 5;125(5):1114-5. doi: 10.1021/ja021230b.