PMID- 12554659
OWN - NLM
STAT- MEDLINE
DCOM- 20030326
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 22
IP  - 3
DP  - 2003 Feb 3
TI  - Histone H1 enhances synergistic activation of the MMTV promoter in chromatin.
PG  - 588-99
AB  - Minichromosomes assembled on the mouse mammary tumor virus (MMTV) promoter in 
      vitro exhibit positioned nucleosomes, one of which covers the binding sites for 
      progesterone receptor (PR) and nuclear factor 1 (NF1). Incorporation of histone 
      H1 into MMTV minichromosomes improves the stability of this nucleosome and 
      decreases basal transcription from the MMTV promoter, as well as its response to 
      either PR or NF1. However, histone H1-containing minichromosomes display better 
      PR binding and support a more efficient synergism between PR and NF1, leading to 
      enhanced transcription initiation. A mutant MMTV promoter lacking positioned 
      nucleosomes does not display enhanced transcriptional synergism in the presence 
      of H1. Binding of PR leads to phosphorylation of H1, which leaves the promoter 
      upon transcription initiation. Thus, H1 assumes a complex and dynamic role in the 
      regulation of the MMTV promoter.
FAU - Koop, Ronald
AU  - Koop R
AD  - Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps-Universitat, 
      E.-Mannkopff-Strasse 2, D-35033 Marburg, Germany.
FAU - Di Croce, Luciano
AU  - Di Croce L
FAU - Beato, Miguel
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Proteins/metabolism
MH  - Chromatin/*genetics/*metabolism
MH  - DNA, Viral
MH  - *DNA-Binding Proteins
MH  - Drosophila melanogaster/physiology
MH  - Gene Expression Regulation, Viral
MH  - Histones/*metabolism
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/*genetics/metabolism
MH  - Mice
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Nucleosomes/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - Receptors, Progesterone/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Swine
MH  - Transcription Factors/metabolism
MH  - *Transcription, Genetic
MH  - Y-Box-Binding Protein 1
PMC - PMC140736
EDAT- 2003/01/30 04:00
MHDA- 2003/03/27 05:00
PMCR- 2004/02/03
CRDT- 2003/01/30 04:00
PHST- 2003/01/30 04:00 [pubmed]
PHST- 2003/03/27 05:00 [medline]
PHST- 2003/01/30 04:00 [entrez]
PHST- 2004/02/03 00:00 [pmc-release]
AID - cdg052 [pii]
AID - 10.1093/emboj/cdg052 [doi]
PST - ppublish
SO  - EMBO J. 2003 Feb 3;22(3):588-99. doi: 10.1093/emboj/cdg052.