PMID- 12556386
OWN - NLM
STAT- MEDLINE
DCOM- 20030210
LR  - 20220311
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 44
IP  - 2
DP  - 2003 Feb
TI  - The corneal stroma is endowed with a significant number of resident dendritic 
      cells.
PG  - 581-9
AB  - PURPOSE: Dendritic cells (DCs) comprise a system of highly efficient 
      antigen-presenting cells (APCs) that initiate immune responses. The purpose of 
      this study was to examine the normal stroma for the presence of DCs and other 
      bone marrow (BM)-derived cells. METHODS: Normal uninflamed corneas of BALB/c and 
      other murine strains were excised, and immunofluorescence single- and 
      double-staining for multiple markers was performed for examination by confocal 
      microscopy. Corneal buttons were placed in culture and immunocytochemistry and 
      flow cytometry performed. RESULTS: MHC class II(+)CD80(+)CD86(+) cells were found 
      in the periphery of the anterior normal stroma. These cells were CD45(+), 
      CD11c(+)CD11b(+) suggesting a BM-derived and monocytic DC lineage. In a 
      surprising finding, significant numbers of MHC class II(-)CD80(-)CD86(-) cells 
      were found in the center of the anterior stroma. These cells were also 
      CD45(+)CD11c(+)CD11b(+) but CD3(-), GR-1(-), keratan sulfate(-), and CD8alpha(-), 
      reflecting an immature precursor phenotype of myeloid DC. In addition to DC 
      subsets in the anterior stroma, a CD11c(-)CD11b(+) population of BM-derived cells 
      was found primarily in the posterior stroma, representing monocytes/macrophages. 
      These cells were rarely present in the anterior third of the normal stroma. 
      Further, CD14(+) precursor-type DCs were found throughout the stroma. These in 
      vivo findings were not strain specific and were confirmed by immunocytochemistry 
      and flow cytometry analyses of cells derived from corneal explants and by 
      transmission electron microscopy. CONCLUSIONS: This study demonstrates that, in 
      addition to the known Langerhans cells in the corneal epithelium, at least three 
      BM-derived cell subsets reside in the normal corneal stroma.
FAU - Hamrah, Pedram
AU  - Hamrah P
AD  - Laboratory of Immunology, Schepens Eye Research Institute, and the Department of 
      Ophthalmology and Massachusetts Eye and Ear Infirmary, Harvard Medical School, 
      Boston, Massachusetts 02114, USA.
FAU - Liu, Ying
AU  - Liu Y
FAU - Zhang, Qiang
AU  - Zhang Q
FAU - Dana, M Reza
AU  - Dana MR
LA  - eng
GR  - R01-EY12963/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antigens, CD)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Cells, Cultured
MH  - Corneal Stroma/*cytology
MH  - Dendritic Cells/*cytology/metabolism/ultrastructure
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Immunophenotyping
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
EDAT- 2003/01/31 04:00
MHDA- 2003/02/11 04:00
CRDT- 2003/01/31 04:00
PHST- 2003/01/31 04:00 [pubmed]
PHST- 2003/02/11 04:00 [medline]
PHST- 2003/01/31 04:00 [entrez]
AID - 10.1167/iovs.02-0838 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2003 Feb;44(2):581-9. doi: 10.1167/iovs.02-0838.