PMID- 12559625
OWN - NLM
STAT- MEDLINE
DCOM- 20030926
LR  - 20231213
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 2
DP  - 2003 Feb
TI  - HCMV glycoprotein US6 mediated inhibition of TAP does not affect HLA-E dependent 
      protection of K-562 cells from NK cell lysis.
PG  - 231-7
AB  - Human cytomegalovirus has evolved multiple strategies to interfere with immune 
      recognition by the host. A variety of mechanisms affect antigen presentation by 
      major histocompatibility complex class I molecules resulting in a reduced class I 
      cell-surface expression. This downregulation is expected to trigger natural 
      killer (NK) cytotoxicity, requiring counteraction by the virus to establish 
      long-term infection. Here we describe that the human cytomegalovirus gpUS6 
      protein, which has been demonstrated to downregulate the expression of human 
      leukocyte antigen (HLA) class I and the presentation of cytotoxic T lymphocyte 
      epitopes by blocking transporter associated with antigen presentation (TAP 
      function), does not affect the ability of HLA-E to inhibit NK cell mediated lysis 
      of K-562 cells by interaction with CD94/NKG2A expressed on NK cells. Cell surface 
      expression and function of HLA-E is not altered although gpUS6 inhibits 
      TAP-dependent peptide transport by 95%. Moreover, HLA-E molecules presenting HLA 
      class I signal sequence-derived peptides are functionally detectable on 
      transfected TAP-deficient RMA-S cells.
FAU - Ulbrecht, Matthias
AU  - Ulbrecht M
AD  - Department Biologie II, Ludwig-Maximilians-Universitat Munchen, Anthropologie und 
      Humangenetik, Munich, Germany.
FAU - Hofmeister, Valeska
AU  - Hofmeister V
FAU - Yuksekdag, Gulnihal
AU  - Yuksekdag G
FAU - Ellwart, Joachim W
AU  - Ellwart JW
FAU - Hengel, Hartmut
AU  - Hengel H
FAU - Momburg, Frank
AU  - Momburg F
FAU - Martinozzi, Silvia
AU  - Martinozzi S
FAU - Reboul, Murielle
AU  - Reboul M
FAU - Pla, Marika
AU  - Pla M
FAU - Weiss, Elisabeth H
AU  - Weiss EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRD1 protein, human)
RN  - 0 (Klrc1 protein, mouse)
RN  - 0 (Klrd1 protein, mouse)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Natural Killer Cell)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TAP1 protein, human)
RN  - 0 (Tap1 protein, mouse)
RN  - 0 (US6 protein, Human cytomegalovirus)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/*antagonists & inhibitors
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, CD/immunology
MH  - Cytotoxicity, Immunologic
MH  - Gene Expression Regulation
MH  - Genes, MHC Class I
MH  - HLA Antigens/biosynthesis/*immunology
MH  - HLA-A2 Antigen/chemistry/immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - K562 Cells
MH  - Killer Cells, Natural/*immunology
MH  - Lectins, C-Type/immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily D
MH  - Peptide Fragments/immunology/metabolism
MH  - RNA-Binding Proteins/*physiology
MH  - Receptors, Immunologic/immunology
MH  - Receptors, Natural Killer Cell
MH  - Recombinant Fusion Proteins/immunology
MH  - Transfection
MH  - Viral Proteins/*physiology
MH  - HLA-E Antigens
EDAT- 2003/02/01 04:00
MHDA- 2003/09/27 05:00
CRDT- 2003/02/01 04:00
PHST- 2003/02/01 04:00 [pubmed]
PHST- 2003/09/27 05:00 [medline]
PHST- 2003/02/01 04:00 [entrez]
AID - S0198885902007887 [pii]
AID - 10.1016/s0198-8859(02)00788-7 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Feb;64(2):231-7. doi: 10.1016/s0198-8859(02)00788-7.