PMID- 12559626
OWN - NLM
STAT- MEDLINE
DCOM- 20030926
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 2
DP  - 2003 Feb
TI  - Specific stimulation of MHC-transgenic mouse T-cell hybridomas with xenogeneic 
      APC.
PG  - 238-44
AB  - From the recombinant human leukocyte antigen (HLA)-DR1/H2-E(k) major 
      histocompatibility complex (MHC) class II-transgenic mice, we have generated two 
      CD4(+) T-cell hybridomas specific for peptides which were derived from human 
      prostatic acid phosphatase (PAP) complexed to the human class II molecule 
      HLA-DR1. Both hybridomas strongly react to PAP-pulsed antigen-presenting cells 
      (APC) from transgenic mice. Interestingly, these hybridomas also responded to PAP 
      antigen presented by HLA-DR1-positive human APC. The species-mismatched T-cell 
      stimulation occurs despite the biologic discordance in participating accessory 
      molecules, which are required for the optimal T-cell-APC interaction. Our results 
      demonstrate various degrees of functional interaction between coreceptors, 
      costimulatory molecules, and integrins, which are expressed on the surface of 
      T-cell hybridomas and heterologous APC.
FAU - Vidovic, Damir
AU  - Vidovic D
AD  - Dendreon Corporation, Seattle, WA 98121, USA. dvidovic@dendreon.com
FAU - Graddis, Thomas J
AU  - Graddis TJ
FAU - Stepan, Lara P
AU  - Stepan LP
FAU - Zaller, Dennis M
AU  - Zaller DM
FAU - Laus, Reiner
AU  - Laus R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, Heterophile)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (H-2 Antigens)
RN  - 0 (H-2E(k) antigen)
RN  - 0 (HLA-DR1 Antigen)
RN  - 0 (Interleukin-2)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (prostatic acid phosphatase)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Acid Phosphatase
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigen-Presenting Cells/*immunology
MH  - Antigens, Heterophile/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Line/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/immunology
MH  - H-2 Antigens/genetics/*immunology
MH  - HLA-DR1 Antigen/genetics/*immunology
MH  - Humans
MH  - Hybridomas/*immunology
MH  - Interleukin-2/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Protein Tyrosine Phosphatases/immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Recombinant Proteins/immunology
MH  - Species Specificity
EDAT- 2003/02/01 04:00
MHDA- 2003/09/27 05:00
CRDT- 2003/02/01 04:00
PHST- 2003/02/01 04:00 [pubmed]
PHST- 2003/09/27 05:00 [medline]
PHST- 2003/02/01 04:00 [entrez]
AID - S0198885902007802 [pii]
AID - 10.1016/s0198-8859(02)00780-2 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Feb;64(2):238-44. doi: 10.1016/s0198-8859(02)00780-2.