PMID- 12559627
OWN - NLM
STAT- MEDLINE
DCOM- 20030926
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 2
DP  - 2003 Feb
TI  - Competition-based cellular peptide binding assays for 13 prevalent HLA class I 
      alleles using fluorescein-labeled synthetic peptides.
PG  - 245-55
AB  - We report the development, validation, and application of competition-based 
      peptide binding assays for 13 prevalent human leukocyte antigen (HLA) class I 
      alleles. The assays are based on peptide binding to HLA molecules on living cells 
      carrying the particular allele. Competition for binding between the test peptide 
      of interest and a fluorescein-labeled HLA class I binding peptide is used as read 
      out. The use of cell membrane-bound HLA class I molecules circumvents the need 
      for laborious biochemical purification of these molecules in soluble form. 
      Previously, we have applied this principle for HLA-A2 and HLA-A3. We now describe 
      the assays for HLA-A1, HLA-A11, HLA-A24, HLA-A68, HLA-B7, HLA-B8, HLA-B14, 
      HLA-B35, HLA-B60, HLA-B61, and HLA-B62. Together with HLA-A2 and HLA-A3, these 
      alleles cover more than 95% of the Caucasian population. Several allele-specific 
      parameters were determined for each assay. Using these assays, we identified 
      novel HLA class I high-affinity binding peptides from HIVpol, p53, PRAME, and 
      minor histocompatibility antigen HA-1. Thus these convenient and accurate 
      peptide-binding assays will be useful for the identification of putative 
      cytotoxic T lymphocyte epitopes presented on a diverse array of HLA class I 
      molecules.
FAU - Kessler, Jan H
AU  - Kessler JH
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, The Netherlands.
FAU - Mommaas, Bregje
AU  - Mommaas B
FAU - Mutis, Tuna
AU  - Mutis T
FAU - Huijbers, Ivo
AU  - Huijbers I
FAU - Vissers, Debby
AU  - Vissers D
FAU - Benckhuijsen, Willemien E
AU  - Benckhuijsen WE
FAU - Schreuder, Geziena M Th
AU  - Schreuder GM
FAU - Offringa, Rienk
AU  - Offringa R
FAU - Goulmy, Els
AU  - Goulmy E
FAU - Melief, Cornelis J M
AU  - Melief CJ
FAU - van der Burg, Sjoerd H
AU  - van der Burg SH
FAU - Drijfhout, Jan W
AU  - Drijfhout JW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Gene Products, pol)
RN  - 0 (HA-1 antigen)
RN  - 0 (HIV Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Oligopeptides)
RN  - 0 (PRAME protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Alleles
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Antigens, Neoplasm/immunology/metabolism
MH  - Binding, Competitive
MH  - Cell Line, Transformed
MH  - Epitopes, T-Lymphocyte/immunology/metabolism
MH  - Gene Products, pol/immunology/metabolism
MH  - *Genes, MHC Class I
MH  - HIV Antigens/immunology/metabolism
MH  - HLA-A Antigens/immunology/metabolism
MH  - HLA-B Antigens/immunology/metabolism
MH  - Histocompatibility Antigens Class I/*immunology/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Inhibitory Concentration 50
MH  - Minor Histocompatibility Antigens/immunology/metabolism
MH  - Molecular Sequence Data
MH  - Oligopeptides/immunology/metabolism
MH  - Peptide Fragments/chemical synthesis/*immunology/metabolism
MH  - Protein Binding
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tumor Suppressor Protein p53/immunology/metabolism
EDAT- 2003/02/01 04:00
MHDA- 2003/09/27 05:00
CRDT- 2003/02/01 04:00
PHST- 2003/02/01 04:00 [pubmed]
PHST- 2003/09/27 05:00 [medline]
PHST- 2003/02/01 04:00 [entrez]
AID - S0198885902007875 [pii]
AID - 10.1016/s0198-8859(02)00787-5 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Feb;64(2):245-55. doi: 10.1016/s0198-8859(02)00787-5.