PMID- 12559710
OWN - NLM
STAT- MEDLINE
DCOM- 20030617
LR  - 20191210
IS  - 0304-4017 (Print)
IS  - 0304-4017 (Linking)
VI  - 111
IP  - 4
DP  - 2003 Feb 27
TI  - Evaluation of tongue inspection and serology for diagnosis of Taenia solium 
      cysticercosis in swine: usefulness of ELISA using purified glycoproteins and 
      recombinant antigen.
PG  - 309-22
AB  - Evaluation of serology using glycoproteins (GPs) purified by preparative 
      isoelectric focusing (pH 8.8) and recombinant chimeric antigen (RecTs) of Taenia 
      solium was carried out using (1) blood samples on filter papers from pigs 
      infected with different doses of eggs of T. solium in Mexico, (2) serum samples 
      from pigs found infected naturally in Vietnam and Ecuador and (3) serum samples 
      from pigs suspected to be infected with T. solium by tongue inspection in 
      Tanzania. Antibody responses (IgG) were detectable in experimentally infected 
      pigs confirmed harbouring 16 or more cysts at necropsy from 30 days after egg 
      inoculation. One of three pigs naturally infected and harbouring 2.5 cysts/kg 
      muscle and most of pigs harbouring=5.0 cysts/kg were also seropositive by ELISA. 
      Although pigs may be infected with other taeniid species such as Taenia 
      hydatigena, pigs harbouring this parasite were negative in ELISA. Approximately, 
      76 and 78% of sera from pigs having nodule(s) in the tongue (positive tongue 
      inspection) were serologically positive by both ELISA and immunoblot, 
      respectively. Furthermore, approximately 34 and 18% of sera from pigs having no 
      nodules in the tongue (negative tongue inspection) were also seropositive by 
      ELISA and immunoblot, respectively. ELISA using the two antigens was more 
      sensitive than immunoblot and reliable for differentiation of pigs infected with 
      cysticerci of T. solium from those either uninfected or infected with other 
      taeniid species. Pigs without nodule by tongue inspection should be checked 
      serologically in endemic areas.
FAU - Sato, M O
AU  - Sato MO
AD  - Department of Parasitology, Asahikawa Medical College, 078-8510, Asahikawa, 
      Japan.
FAU - Yamasaki, H
AU  - Yamasaki H
FAU - Sako, Y
AU  - Sako Y
FAU - Nakao, M
AU  - Nakao M
FAU - Nakaya, K
AU  - Nakaya K
FAU - Plancarte, A
AU  - Plancarte A
FAU - Kassuku, A A
AU  - Kassuku AA
FAU - Dorny, P
AU  - Dorny P
FAU - Geerts, S
AU  - Geerts S
FAU - Benitez-Ortiz, W
AU  - Benitez-Ortiz W
FAU - Hashiguchi, Y
AU  - Hashiguchi Y
FAU - Ito, A
AU  - Ito A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Parasitol
JT  - Veterinary parasitology
JID - 7602745
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Glycoproteins)
RN  - 0 (Helminth Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/blood
MH  - Antigens, Helminth/*immunology
MH  - Cysticercosis/*diagnosis/immunology/parasitology/*veterinary
MH  - Ecuador
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/immunology
MH  - Helminth Proteins/immunology
MH  - Mexico
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/immunology/parasitology/*pathology
MH  - Taenia solium/immunology/*isolation & purification
MH  - Tongue/*pathology
MH  - Vietnam
EDAT- 2003/02/01 04:00
MHDA- 2003/06/18 05:00
CRDT- 2003/02/01 04:00
PHST- 2003/02/01 04:00 [pubmed]
PHST- 2003/06/18 05:00 [medline]
PHST- 2003/02/01 04:00 [entrez]
AID - S0304401702003837 [pii]
AID - 10.1016/s0304-4017(02)00383-7 [doi]
PST - ppublish
SO  - Vet Parasitol. 2003 Feb 27;111(4):309-22. doi: 10.1016/s0304-4017(02)00383-7.