PMID- 12561366
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20181130
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 19
IP  - 4
DP  - 2002 Dec
TI  - [Characteristics of tenocyte adhesion to biologically-modified surface of 
      polymer].
PG  - 633-8
AB  - In this study we examined the in vitro characteristics of tenocyte adhesion to 
      biologically-modified surface of polymer. Polylactic-co-glycolic acid (PLGA) 
      85/15 films were prepared by a solvent-casting technique. Each film was adhered 
      onto the bottom of a chamber. The film was precoated with poly-D-lysine (PDL), 
      and then coated with serum-free F12 medium containing various concentrations of 
      fibronectin (FN), type I collagen (CN I), and insulin-like growth factor1 
      (IGF-1). The monoclonal antibodies (to FN and to CN I) with various dilutions 
      were used to inhibit attachment of tenocytes to surface precoated with FN or CN 
      I. Human embryonic tendon cells (HETCs) and transformed human embryonic tendon 
      cells (THETCs) were used as the seeding cells. The system used for the 
      measurement of adhesion force was the micropipette aspiration experiment system. 
      The micropipette was manipulated to aspirate a small portion of the tenocyte body 
      by using a small aspiration pressure. Then the pipette was pulled away from the 
      adhesion area by micromanipulation. The minimum force required to detach the 
      tenocyte from the substrate was defined as the adhesion force. The results showed 
      that modification of FN or CN I by precoating significantly enhanced attachment 
      of tenocytes to surface of polymer (P < 0.05). As antibodies to FN or CN I were 
      added to a polymer film precoated with FN or CN I, the adhesion force decreased 
      significantly (P < 0.05). We concluded that the specific adhesion forces of 
      tenocytes to extracellular matrix adhesion proteins (FN and CN I) had coordinated 
      action and showed good dependence on their precoating concentrations, and were 
      inhibited by the antibodies to these adhesion proteins. Films precoated with 
      IGF-1 strongly accelerated the adhesion of tenocytes to polymer. These results 
      indicate that the specific adhesion of tenocytes to polymer can be promoted by 
      coating extracellular matrix adhesive proteins and insulin-like growth factor1. 
      It is of great importance to construct tissue-engineered tendon.
FAU - Qin, Tingwu
AU  - Qin T
AD  - Department of Orthopedic Surgery, West China Hospital of Sichuan University, 
      Chengdu 610041. qtw@mcwcums.com
FAU - Yang, Zhiming
AU  - Yang Z
FAU - Xie, Huiqi
AU  - Xie H
FAU - Li, Hong
AU  - Li H
FAU - Qin, Jian
AU  - Qin J
FAU - Wu, Zezhi
AU  - Wu Z
FAU - Xu, Shirong
AU  - Xu S
FAU - Cai, Shaoxi
AU  - Cai S
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Biocompatible Materials)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Growth Substances)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 25104-18-1 (Polylysine)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - Cell Adhesion/drug effects/physiology
MH  - Cells, Cultured
MH  - Extracellular Matrix Proteins/pharmacology
MH  - Growth Substances/pharmacology
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polylysine/pharmacology
MH  - Polymers/*chemistry
MH  - Tendons/cytology/embryology/*physiology
MH  - Tissue Engineering
EDAT- 2003/02/04 04:00
MHDA- 2003/06/19 05:00
CRDT- 2003/02/04 04:00
PHST- 2003/02/04 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/02/04 04:00 [entrez]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2002 Dec;19(4):633-8.