PMID- 12562328
OWN - NLM
STAT- MEDLINE
DCOM- 20030407
LR  - 20240413
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 108
IP  - 2
DP  - 2003 Feb
TI  - Administration of exogenous interleukin-18 and interleukin-12 prevents the 
      induction of oral tolerance.
PG  - 196-203
AB  - Interleukin-18 (IL-18), a pro-inflammatory member of the IL-1 family, has been 
      associated with autoimmunity and allergic disease. This type of pathology is 
      thought to be the result of a defect in immunological tolerance and is often 
      observed in inflammatory disorders of the gut such as coeliac disease, Crohn's 
      disease and ulcerative colitis. IL-18 has been implicated in a number of mucosal 
      immune disorders, where it synergizes with IL-12 to induce the production of 
      interferon-gamma (IFN-gamma). We have therefore investigated the effects of IL-18 
      and IL-12 administration on the induction of oral tolerance to ovalbumin. The 
      suppression of specific Ig G2a production, delayed-type hypersensitivity 
      responses and IFN-gamma production by antigen-specific T cells were all abrogated 
      by the presence of exogenous IL-12 and IL-18, suggesting that oral tolerance was 
      broken. The expression of the co-stimulatory molecule CD80 on dendritic cells was 
      also shown to be increased by this combination of cytokines. As dendritic cells 
      are thought to be of major importance in the induction of tolerance, this 
      suggests a mechanism by which tolerance to mucosal antigens may be broken in 
      vivo.
FAU - Eaton, Alfred D
AU  - Eaton AD
AD  - Division of Immunology, Infection & Inflammation, University of Glasgow, Western 
      Infirmary, Glasgow, UK.
FAU - Xu, Damo
AU  - Xu D
FAU - Garside, Paul
AU  - Garside P
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-18)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - Immune Tolerance/*immunology
MH  - Immunity, Mucosal
MH  - Immunoglobulin G/biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-12/*immunology
MH  - Interleukin-18/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
PMC - PMC1782890
EDAT- 2003/02/04 04:00
MHDA- 2003/04/08 05:00
PMCR- 2004/02/01
CRDT- 2003/02/04 04:00
PHST- 2003/02/04 04:00 [pubmed]
PHST- 2003/04/08 05:00 [medline]
PHST- 2003/02/04 04:00 [entrez]
PHST- 2004/02/01 00:00 [pmc-release]
AID - 1570 [pii]
AID - 10.1046/j.1365-2567.2003.01570.x [doi]
PST - ppublish
SO  - Immunology. 2003 Feb;108(2):196-203. doi: 10.1046/j.1365-2567.2003.01570.x.