PMID- 12562398
OWN - NLM
STAT- MEDLINE
DCOM- 20030407
LR  - 20190513
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 131
IP  - 2
DP  - 2003 Feb
TI  - Antigens shared by malignant plasma cells and normal B cells may be involved in 
      graft versus myeloma.
PG  - 340-6
AB  - Cytotoxic T cells play an important role in graft-versus-host-disease (GvHD) and 
      graft-versus-leukaemia/myeloma, which may occur in patients treated with an 
      allogeneic stem cell transplantation (ASCT). Here, we describe the selection of a 
      myeloma reactive CD4+ cytotoxic T cell-line (CTL) and two CD4+ clones from this 
      CTL. The CTL was generated from the blood from a patient with multiple myeloma 
      (MM) with graft versus myeloma/GvHD, following an ASCT. The CTL was stimulated 
      using irradiated peripheral blood mononuclear cells and EBV transformed B cells 
      from the myeloma patient (EBVp), both of which were obtained prior to ASCT. Both 
      the CTL and the two T cell clones specifically lysed EBVp and secreted IFN-gamma 
      after coculture with EBVp and autologous myeloma tumour cells in a class II 
      restricted fashion. These results show that myeloma tumour cells and autologous B 
      cells present a common polymorphic peptide that functions as a target for graft 
      derived cytotoxic T cells. Identification of these proteins will give insight 
      into the relationship between graft versus myeloma (GvM) and GvHD and may provide 
      immunotherapeutical targets in the treatment of MM.
FAU - Holloway, P A
AU  - Holloway PA
AD  - Department of Haematology, UMCU, Utrecht, The Netherlands.
FAU - Kaldenhoven, N
AU  - Kaldenhoven N
FAU - Kok-Schoemaker, H M
AU  - Kok-Schoemaker HM
FAU - Van Kessel, B
AU  - Van Kessel B
FAU - Van Blokland, W T M
AU  - Van Blokland WT
FAU - Bloem, A C
AU  - Bloem AC
FAU - Lokhorst, H M
AU  - Lokhorst HM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antigens, Surface)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Surface/analysis
MH  - B-Lymphocytes/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Graft vs Host Reaction/*immunology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Herpesvirus 4, Human
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*immunology
MH  - Plasma Cells/*immunology
PMC - PMC1808635
EDAT- 2003/02/04 04:00
MHDA- 2003/04/08 05:00
PMCR- 2004/02/01
CRDT- 2003/02/04 04:00
PHST- 2003/02/04 04:00 [pubmed]
PHST- 2003/04/08 05:00 [medline]
PHST- 2003/02/04 04:00 [entrez]
PHST- 2004/02/01 00:00 [pmc-release]
AID - 2067 [pii]
AID - 10.1046/j.1365-2249.2003.02067.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2003 Feb;131(2):340-6. doi: 10.1046/j.1365-2249.2003.02067.x.