PMID- 12563260
OWN - NLM
STAT- MEDLINE
DCOM- 20030326
LR  - 20221109
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 4
IP  - 3
DP  - 2003 Mar
TI  - Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 
      function.
PG  - 287-94
AB  - Phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog (PTEN) 
      phosphatase serve essential functions in the regulation of cell growth, 
      differentiation and survival by modulating intracellular 
      phosphatidylinositol-3,4,5-trisphosphate (PI-3,4,5-P3) concentrations. Here we 
      show that the conditional deletion of Pten in B cells led to the preferential 
      generation of marginal zone (MZ) B cells and B1 cells. PTEN-deficient B cells 
      were hyperproliferative in response to mitogenic stimuli, and exhibited a lower 
      threshold for activation through the B cell antigen receptor. Inactivation of 
      PTEN rescued germinal center, MZ B and B1 cell formation in CD19-/- mice, arguing 
      that recruitment and activation of PI3K are the dominant roles for CD19 in these 
      B cell subpopulations. These findings establish the central role of PI-3,4,5-P3 
      regulation in the differentiation of peripheral B cell subsets.
FAU - Anzelon, Amy N
AU  - Anzelon AN
AD  - Division of Biological Sciences, University of California, San Diego, 9500 Gilman 
      Drive, MC 0322, La Jolla, CA 92093, USA.
FAU - Wu, Hong
AU  - Wu H
FAU - Rickert, Robert C
AU  - Rickert RC
LA  - eng
GR  - AI41649/AI/NIAID NIH HHS/United States
GR  - GM07246/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030203
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 0 (Antigens, CD19)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
SB  - IM
MH  - Animals
MH  - Antigens, CD19/*metabolism
MH  - B-Lymphocytes/*metabolism
MH  - Cell Differentiation/physiology
MH  - Mice
MH  - PTEN Phosphohydrolase
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphoric Monoester Hydrolases/deficiency/*metabolism
MH  - Spleen/metabolism
MH  - Tumor Suppressor Proteins/deficiency/*metabolism
EDAT- 2003/02/04 04:00
MHDA- 2003/03/27 05:00
CRDT- 2003/02/04 04:00
PHST- 2002/11/04 00:00 [received]
PHST- 2003/01/13 00:00 [accepted]
PHST- 2003/02/04 04:00 [pubmed]
PHST- 2003/03/27 05:00 [medline]
PHST- 2003/02/04 04:00 [entrez]
AID - ni892 [pii]
AID - 10.1038/ni892 [doi]
PST - ppublish
SO  - Nat Immunol. 2003 Mar;4(3):287-94. doi: 10.1038/ni892. Epub 2003 Feb 3.