PMID- 12563424
OWN - NLM
STAT- MEDLINE
DCOM- 20040914
LR  - 20190922
IS  - 1516-3180 (Print)
IS  - 1516-3180 (Linking)
VI  - 120
IP  - 6
DP  - 2002 Nov 1
TI  - Addition of exogenous cytokines in mixed lymphocyte culture for selecting related 
      donors for bone marrow transplantation.
PG  - 175-9
AB  - CONTEXT: Mixed lymphocyte culturing has led to conflicting opinions regarding the 
      selection of donors for bone marrow transplantation. The association between a 
      positive mixed lymphocyte culture and the development of graft-versus-host 
      disease (GVHD) is unclear. The use of exogenous cytokines in mixed lymphocyte 
      cultures could be an alternative for increasing the sensitivity of culture tests. 
      OBJECTIVE: To increase the sensitivity of mixed lymphocyte cultures between donor 
      and recipient human leukocyte antigen (HLA) identical siblings, using exogenous 
      cytokines, in order to predict post-transplantation GVHD and/or rejection. TYPE 
      OF STUDY: Prospective study. SETTING: Bone Marrow Transplantation Unit, 
      Universidade Estadual de Campinas. PARTICIPANTS: Seventeen patients with 
      hematological malignancies and their respective donors selected for bone marrow 
      transplantation procedures. PROCEDURES: Standard and modified mixed lymphocyte 
      culturing by cytokine supplementation was carried out using donor and recipient 
      cells typed for HLA. MAIN MEASUREMENTS: Autologous and allogenic responses in 
      mixed lymphocyte cultures after the addition of IL-4 or IL-2. RESULTS: In 
      comparison with the standard method, average responses in the modified mixed 
      lymphocyte cultures increased by a factor of 2.0 using IL-4 (p < 0.001) and 6.4 
      using IL-2 (p < 0.001), for autologous donor culture responses. For 
      donor-versus-recipient culture responses, the increase was by a factor of 1.9 
      using IL-4 (p < 0.001) and 4.1 using IL-2 (p < 0.001). For donor-versus-unrelated 
      culture responses, no significant increase was observed using IL-4, and a mean 
      response inhibition of 20% was observed using IL-2 (p < 0.001). Neither of the 
      cytokines produced a significant difference in the unrelated control versus 
      recipient cell responses. CONCLUSION: IL-4 supplementation was the best for 
      increasing the mixed lymphocyte culture sensitivity. However, IL-4 also increased 
      autologous responses, albeit less intensively than IL-2. Thus, with this loss of 
      specificity we believe that it is not worth modifying the traditional mixed 
      lymphocyte culture method, even with IL-4 addition.
FAU - Visentainer, Jeane Eliete Laguila
AU  - Visentainer JE
AD  - Universidade Estadual de Campinas, Campinas, Sao Paulo, Brazil. 
      jelvisentainer@ibest.com.br
FAU - Lieber, Sofia Rocha
AU  - Lieber SR
FAU - Persoli, L gia Beatriz Lopes
AU  - Persoli Lg
FAU - Vigorito, Afonso Celso
AU  - Vigorito AC
FAU - Aranha, Francisco Jos Penteado
AU  - Aranha FJ
FAU - Souza, C rmino Antonio de
AU  - Souza Cr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030122
PL  - Brazil
TA  - Sao Paulo Med J
JT  - Sao Paulo medical journal = Revista paulista de medicina
JID - 100897261
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Interleukin-2)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Adult
MH  - Bone Marrow Transplantation/*immunology
MH  - Female
MH  - Graft vs Host Disease/immunology/prevention & control
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-2/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Lymphocyte Culture Test, Mixed/*methods
MH  - Male
MH  - Prospective Studies
MH  - Sensitivity and Specificity
EDAT- 2003/02/04 04:00
MHDA- 2004/09/15 05:00
CRDT- 2003/02/04 04:00
PHST- 2003/02/04 04:00 [pubmed]
PHST- 2004/09/15 05:00 [medline]
PHST- 2003/02/04 04:00 [entrez]
AID - S1516-31802002000600004 [pii]
AID - 10.1590/s1516-31802002000600004 [doi]
PST - ppublish
SO  - Sao Paulo Med J. 2002 Nov 1;120(6):175-9. doi: 10.1590/s1516-31802002000600004. 
      Epub 2003 Jan 22.