PMID- 12563736
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20061115
IS  - 1000-7423 (Print)
IS  - 1000-7423 (Linking)
VI  - 17
IP  - 6
DP  - 1999
TI  - [Finding of new FCC1/HN antigenic expressed sequence tag(ESTs) of Plasmodium 
      falciparum].
PG  - 363-6
AB  - AIM: To sequence the strong positive clones obtained by immuno-screening of 
      Plasmodium falciparum FCC1/HN lambda gt11 cDNA expression library, and to 
      elucidate the antigenic expressed sequence tags through sequencing the cDNA 
      insert of these positive clones, and new antigenic ESTs could serve as a resource 
      to pursue their corresponding antigen genes. METHODS: cDNA inserts of positive 
      lambda gt11 phage clones were amplified by PCR. The PCR products, after 
      purification, were cloned into the M13 mp18 sequencing vector. Single-stranded 
      M13 DNA was prepared and sequenced. Then the acquired sequences were compared in 
      homologies with EMBL/GenBank database on the PC/GENE software system and searched 
      in NCBI (National Center for Biotechnology Information) GenBank using BLAST 
      (Basic Local Alignment Search Tool) commond. RESULTS: Sequence C03 was part of 
      the known P. falciparum antigenic heat shock protein 70 (Pfhsp70) gene, while the 
      other 5 sequences were new P. falciparum antigenic expressed sequence tags 
      (ESTs). CONCLUSION: The 5 new antigenic ESTs generated could serve as the 
      breaking through points in our efforts to find out new P. falciparum antigen 
      genes.
FAU - Yan, Z
AU  - Yan Z
AD  - Department of Tropical Diseases, Institute of Tropical Medicine, The First 
      Military Medical University, Guangzhou, 510515.
FAU - Xie, Y
AU  - Xie Y
FAU - Li, M
AU  - Li M
FAU - Wang, P
AU  - Wang P
FAU - Wang, Y
AU  - Wang Y
FAU - Bi, H
AU  - Bi H
FAU - Li, Y
AU  - Li Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
JT  - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of 
      parasitology & parasitic diseases
JID - 8709992
RN  - 0 (Antigens, Protozoan)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Antigens, Protozoan/*genetics
MH  - Base Sequence
MH  - DNA, Complementary/*genetics
MH  - *Expressed Sequence Tags
MH  - Gene Library
MH  - Molecular Sequence Data
MH  - Plasmodium falciparum/*immunology
MH  - Sequence Analysis, DNA
EDAT- 2003/02/05 04:00
MHDA- 2003/06/19 05:00
CRDT- 2003/02/05 04:00
PHST- 2003/02/05 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/02/05 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(6):363-6.