PMID- 12563770
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20041117
IS  - 1000-7423 (Print)
IS  - 1000-7423 (Linking)
VI  - 17
IP  - 4
DP  - 1999
TI  - Establishment of immunoglobulin M(IgM)-immunosorbent agglutination assay (ISAGA) 
      for diagnosis of toxoplasmosis.
PG  - 225-7
AB  - AIM: To establish an immunosorbent agglutination assay (ISAGA) for detection of 
      IgM antibodies against Toxoplasma gondii. METHODS: In the ISAGA, wells of 
      microtiter plates were coated with anti-human IgM antibodies and sealed with 1% 
      bovine serum albumin. After the test sera were added and incubated, the plates 
      were washed, T. gondii tachyzoite antigen suspension was added, and incubated 
      overnight at 37 degrees C. The ISAGA results were evaluated by comparing with 
      those detected by Danish ISAGA and ELISA and those detected by slide enzyme 
      immunoassay (S-EIA). RESULTS: Forty-four sera from Danish pregnant women were 
      tested by the IgM-ISAGA, 41(93.2%) were consistent with the Danish results. 
      Sixty-seven sera from Danish and Shanghai pregnant women were detected by 
      IgM-IgM-ISAGA and S-EIA, the total consistency rate was 92.5%. A significant 
      correlation was found between the titers of the ISAGA and S-EIA, with a Pearson 
      correlation coefficient of 0.589(P < 0.001). The titers of ISAGA were eighteen 
      times higher than those of S-EIA. This method enables the detection of IgM 
      antibodies as low as approximately 0.08 IU/ml. CONCLUSION: The IgM-ISAGA is 
      therefore sensitive, specific, easy to perform, and is useful for mass screening 
      and diagnosing recent Toxoplasma infection or reactivation.
FAU - Zhang, S
AU  - Zhang S
AD  - Shanghai Detecting Center for Toxoplasmosis, Shanghai Institute of Parasitic 
      Disease Control and Rsesarch, Shanghai 200336.
FAU - Wei, M
AU  - Wei M
FAU - Zhao, H
AU  - Zhao H
FAU - Shi, G
AU  - Shi G
LA  - eng
PT  - Journal Article
PL  - China
TA  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
JT  - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of 
      parasitology & parasitic diseases
JID - 8709992
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Agglutination Tests
MH  - Animals
MH  - Female
MH  - Humans
MH  - Immunoglobulin M/*blood
MH  - Immunosorbent Techniques
MH  - Pregnancy
MH  - Pregnancy Complications, Parasitic/*diagnosis/immunology
MH  - Sensitivity and Specificity
MH  - Toxoplasma/*immunology
MH  - Toxoplasmosis/*diagnosis
EDAT- 2003/02/05 04:00
MHDA- 2003/06/19 05:00
CRDT- 2003/02/05 04:00
PHST- 2003/02/05 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/02/05 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):225-7.