PMID- 12564628 OWN - NLM STAT- MEDLINE DCOM- 20030304 LR - 20190607 IS - 0018-0661 (Print) IS - 0018-0661 (Linking) VI - 137 IP - 1 DP - 2002 TI - Physical mapping of the 5S and 45S rDNA in teosintes. PG - 16-9 AB - The physical locations of the 5S and 45S rDNA sequences were examined in three types of teosinte, Zea mays ssp. mexicana (2n = 20), Zea diploperennis (2n = 20) and Zea perennis (2n = 40) by biotinylated fluorescence in situ hybridization (FISH). The tested materials only showed one hybridization site of 5S rDNA on their genomes, but they were different in the position of the signals. The hybridization site of Zea mays ssp. mexicana was located on the long arm of chromosome 2, indicating that it is the same as the cultivated maize in the position of 5S rDNA, while the sites of Zea diploperennis and Zea perennis were on the short arms of other chromosomes. For 45S rDNA, one hybridization site was detected at secondary constriction region of the satellite chromosomes in Zea mays ssp. mexicana and Zea diploperennis, while in Zea perennis, besides the site located at the secondary constriction region, a second site on the short arm of another chromosome pair was observed. Our results provide additional evidence for Zea mays ssp. mexicana being a subspecies of Zea mays. FAU - Han, Y H AU - Han YH AD - The Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan Hubei, P.R. China. FAU - Li, L J AU - Li LJ FAU - Song, Y C AU - Song YC FAU - Li, Z Y AU - Li ZY FAU - Xiong, Z Y AU - Xiong ZY FAU - Li, D Y AU - Li DY LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Hereditas JT - Hereditas JID - 0374654 RN - 0 (DNA Probes) RN - 0 (DNA, Ribosomal) SB - IM MH - DNA Probes MH - DNA, Ribosomal/*genetics MH - In Situ Hybridization, Fluorescence MH - Phylogeny MH - Zea mays/*genetics EDAT- 2003/02/05 04:00 MHDA- 2003/03/05 04:00 CRDT- 2003/02/05 04:00 PHST- 2003/02/05 04:00 [pubmed] PHST- 2003/03/05 04:00 [medline] PHST- 2003/02/05 04:00 [entrez] AID - 10.1034/j.1601-5223.2002.1370103.x [doi] PST - ppublish SO - Hereditas. 2002;137(1):16-9. doi: 10.1034/j.1601-5223.2002.1370103.x.