PMID- 12564761
OWN - NLM
STAT- MEDLINE
DCOM- 20030402
LR  - 20191106
IS  - 0919-6544 (Print)
IS  - 0919-6544 (Linking)
VI  - 22
IP  - 4
DP  - 2002 Dec
TI  - The constitutive and inducible expression of Nurr1, a key regulator of 
      dopaminergic neuronal differentiation, in human neural and non-neural cell lines.
PG  - 219-32
AB  - Nur-related factor 1 (Nurr1), nerve growth factor-induced gene B (NGFI-B) and 
      neuron-derived orphan receptor-1 (NOR-1) constitute the orphan nuclear receptor 
      subfamily of transcription factors. Previous studies showed that midbrain 
      dopaminergic neuronal precursor cells failed to differentiate in Nurr1-deficient 
      mice. To investigate a role of Nurr1 in human neuronal function, Nurr1 mRNA 
      expression was studied in human neural cell lines by RT-PCR and northern blot 
      analysis. Nurr1, NGFI-B and NOR-1 mRNA were coexpressed in all human neural and 
      nonneural cell lines under the serum-containing culture condition, except for 
      SK-N-SH neuroblastoma, in which Nurr1 mRNA was undetectable. The levels of Nurr1, 
      NGFI-B and NOR-1 mRNA were elevated markedly in NTera2 teratocarcinoma-derived 
      neurons (NTera2-N), a model of differentiated human neurons, following a 1.5 or 3 
      h-exposure to 1 mM dibutyryl cyclic AMP or 100 nm phorbol 12-myristate 
      13-acetate. NGFI-B mRNA levels were also elevated in NTera2-N cells by exposure 
      to 100 ng/mL brain-derived neurotrophic factor (BDNF). To identify Nurr1-target 
      genes, the mRNA expression of 27 genes potentially involved in dopaminergic 
      neuronal differentiation and survival, including BDNF, glia-derived neurotrophic 
      factor, their receptors, tyrosine hydroxylase and alpha-synuclein, were studied 
      in HEK293 cells following overexpression of Nurr1. None of these genes examined, 
      however, showed significant changes. These results indicate that Nurr1, NGFI-B 
      and NOR-1 mRNA are expressed constitutively in various human neural and 
      non-neural cell lines under the serum-containing culture condition, and their 
      levels are up-regulated in human neurons by activation of protein kinase A or 
      protein kinase C pathway, although putative coactivators expressed in 
      dopaminergic neuronal precursor cells might be required for efficient 
      transcriptional activation of Nurr1-target genes.
FAU - Satoh, Jun-ichi
AU  - Satoh J
AD  - Department of Immunology, National Institute of Neuroscience, NCNP, Kodaira, 
      Tokyo, Japan. satoj@ncnp.go.jp
FAU - Kuroda, Yasuo
AU  - Kuroda Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Neuropathology
JT  - Neuropathology : official journal of the Japanese Society of Neuropathology
JID - 9606526
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Activators)
RN  - 0 (NR4A1 protein, human)
RN  - 0 (NR4A2 protein, human)
RN  - 0 (NR4A3 protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nr4a1 protein, mouse)
RN  - 0 (Nr4a2 protein, mouse)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 1)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Transcription Factors)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Blotting, Northern
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Bucladesine/pharmacology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects/genetics
MH  - DNA-Binding Proteins/*biosynthesis/drug effects
MH  - Enzyme Activators/pharmacology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Nerve Tissue Proteins/biosynthesis/drug effects
MH  - Neurons/cytology/drug effects/*physiology
MH  - Nuclear Receptor Subfamily 4, Group A, Member 1
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2
MH  - RNA, Messenger/analysis
MH  - Receptors, Cytoplasmic and Nuclear
MH  - Receptors, Steroid
MH  - Receptors, Thyroid Hormone
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription Factors/*biosynthesis/drug effects
MH  - Tumor Cells, Cultured
EDAT- 2003/02/05 04:00
MHDA- 2003/04/04 05:00
CRDT- 2003/02/05 04:00
PHST- 2003/02/05 04:00 [pubmed]
PHST- 2003/04/04 05:00 [medline]
PHST- 2003/02/05 04:00 [entrez]
AID - 10.1046/j.1440-1789.2002.00460.x [doi]
PST - ppublish
SO  - Neuropathology. 2002 Dec;22(4):219-32. doi: 10.1046/j.1440-1789.2002.00460.x.