PMID- 12564767 OWN - NLM STAT- MEDLINE DCOM- 20030402 LR - 20220330 IS - 0919-6544 (Print) IS - 0919-6544 (Linking) VI - 22 IP - 4 DP - 2002 Dec TI - Ischemic rat brain extracts induce human marrow stromal cell growth factor production. PG - 275-9 AB - Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these functional benefits are derived in part from hMSC production of growth and trophic factors. Quantitative sandwich enzyme-linked immunosorbent assay (ELISA) of hMSCs cultured with normal and MCAo brain extracts were performed. hMSCs cultured in supernatant derived from ischemic brain extracts increased production of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). These neurotrophins and angiogenic growth factors increased in a post-ischemia time-dependent manner. The hMSC capacity to increase expression of growth and trophic factors may be the key to the benefit provided by transplanted hMSCs in the ischemic brain. FAU - Chen, Xiaoguang AU - Chen X AD - Department of Neurology, Division of Hematology/Medical Oncology, Henry Ford Health Sciences Center, Detroit, USA. FAU - Li, Yi AU - Li Y FAU - Wang, Lei AU - Wang L FAU - Katakowski, Mark AU - Katakowski M FAU - Zhang, Lijie AU - Zhang L FAU - Chen, Jieli AU - Chen J FAU - Xu, Yongxian AU - Xu Y FAU - Gautam, Subhash C AU - Gautam SC FAU - Chopp, Michael AU - Chopp M LA - eng GR - P01 NS23393/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - Australia TA - Neuropathology JT - Neuropathology : official journal of the Japanese Society of Neuropathology JID - 9606526 RN - 0 (Growth Substances) RN - 0 (Tissue Extracts) SB - IM MH - Animals MH - Bone Marrow Cells/*drug effects/metabolism MH - Brain Ischemia/metabolism MH - Cell Transplantation MH - Cells, Cultured MH - Enzyme-Linked Immunosorbent Assay MH - Growth Substances/analysis/*metabolism MH - Humans MH - Rats MH - Stromal Cells/*drug effects/metabolism MH - Time Factors MH - Tissue Extracts/*pharmacology EDAT- 2003/02/05 04:00 MHDA- 2003/04/04 05:00 CRDT- 2003/02/05 04:00 PHST- 2003/02/05 04:00 [pubmed] PHST- 2003/04/04 05:00 [medline] PHST- 2003/02/05 04:00 [entrez] AID - 10.1046/j.1440-1789.2002.00450.x [doi] PST - ppublish SO - Neuropathology. 2002 Dec;22(4):275-9. doi: 10.1046/j.1440-1789.2002.00450.x.