PMID- 12565195
OWN - NLM
STAT- MEDLINE
DCOM- 20030328
LR  - 20220330
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 138
IP  - 3
DP  - 2003 Mar 3
TI  - Zearalenone induces male germ cell apoptosis in rats.
PG  - 185-92
AB  - Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin, is known to cause 
      toxicity of testis in male rats. To investigate whether apoptosis is involved in 
      ZEA-induced testicular toxicity and to identify the stage and target germ cell 
      type, 10-week-old Sprague-Dawley male rats were treated with a single 
      intraperitoneal (i.p.) dose of ZEA (5 mg/kg) and euthanized at 3, 6, 12, 24, or 
      48 h subsequently. Histopathologically, germ cell degeneration was found at 
      stages I-VI 12 h after dosing. Degenerating germ cells were shown to undergo 
      apoptosis as revealed by in situ terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL). The frequency of TUNEL-labeled germ cells 
      increased in a stage-specific manner, the peak frequency gradually progressing at 
      stages I-VI of seminiferous tubules with time after dosing, suggesting that the 
      damaged germ cells, especially spermatogonia and spermatocytes, gradually 
      underwent the processes leading to apoptosis. DNA laddering on gel 
      electrophoresis was apparent 12 h after dosing. The results demonstrated that a 
      single dose of ZEA induces testicular germ cell apoptosis in a time-dependent and 
      stage-specific pattern. This study has established that apoptosis is the 
      principal mechanism contributing to germ cell depletion and testicular atrophy 
      following ZEA exposure.
CI  - Copyright 2002 Elsevier Science Ireland Ltd.
FAU - Kim, Il-Hwan
AU  - Kim IH
AD  - Korea Institute of Toxicology, PO Box 123, 100 Jang-dong, Yuseong-gu, Daejeon 
      305-600, South Korea.
FAU - Son, Hwa-Young
AU  - Son HY
FAU - Cho, Sung-Whan
AU  - Cho SW
FAU - Ha, Chang-Su
AU  - Ha CS
FAU - Kang, Boo-Hyon
AU  - Kang BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - DNA Fragmentation/drug effects
MH  - Electrophoresis, Agar Gel
MH  - Estrogens, Non-Steroidal/*toxicity
MH  - Histocytochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/pathology
MH  - Spermatocytes/*drug effects/pathology
MH  - Spermatogonia/*drug effects/pathology
MH  - Zearalenone/*toxicity
EDAT- 2003/02/05 04:00
MHDA- 2003/03/29 05:00
CRDT- 2003/02/05 04:00
PHST- 2003/02/05 04:00 [pubmed]
PHST- 2003/03/29 05:00 [medline]
PHST- 2003/02/05 04:00 [entrez]
AID - S0378427402004058 [pii]
AID - 10.1016/s0378-4274(02)00405-8 [doi]
PST - ppublish
SO  - Toxicol Lett. 2003 Mar 3;138(3):185-92. doi: 10.1016/s0378-4274(02)00405-8.