PMID- 12568372
OWN - NLM
STAT- MEDLINE
DCOM- 20030728
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 983
IP  - 1-2
DP  - 2003 Jan 3
TI  - Elevated-temperature ultrahigh-pressure liquid chromatography using very small 
      polybutadiene-coated nonporous zirconia particles.
PG  - 83-9
AB  - Capillary columns packed with small diameter particles typically lead to low 
      permeability and long separation times in high-performance liquid chromatography. 
      Ultrahigh pressures (>10,000 p.s.i.; 1 p.s.i. is identical with 6,894.76 Pa) can 
      be used to overcome the limitations that small particles impose. 
      Ultrahigh-pressure liquid chromatography (UHPLC) has demonstrated great potential 
      for high-speed and high-efficiency separations. Decreasing the viscosity of the 
      mobile phase by elevating the temperature could additionally reduce the pressure 
      drop and facilitate the use of longer columns or smaller particles to achieve 
      even higher total plate numbers. For this reason, we investigated the use of 
      elevated temperatures in UHPLC. Water-resistant, flexible heater tape covered 
      with insulation was used to provide the desired heat to the column. 
      Polybutadiene-coated 1 microm nonporous zirconia particles were used because of 
      their chemical stability at elevated temperature. A column efficiency as high as 
      420,000 plates m(-1) was obtained. The effects of temperature and pressure on the 
      separation of parabens were investigated. Separation of five herbicides was 
      completed in 60 s using 26,000 p.s.i. and 90 degrees C.
FAU - Xiang, Yanqiao
AU  - Xiang Y
AD  - Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, 
      84602-5700, USA.
FAU - Yan, Bingwen
AU  - Yan B
FAU - Yue, Bingfang
AU  - Yue B
FAU - McNeff, Clayton V
AU  - McNeff CV
FAU - Carr, Peter W
AU  - Carr PW
FAU - Lee, Milton L
AU  - Lee ML
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Butadienes)
RN  - 0 (Elastomers)
RN  - 0 (Polymers)
RN  - 9003-17-2 (polybutadiene)
RN  - C6V6S92N3C (Zirconium)
RN  - S38N85C5G0 (zirconium oxide)
SB  - IM
MH  - Butadienes/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Elastomers
MH  - Hot Temperature
MH  - Polymers/*chemistry
MH  - Stereoisomerism
MH  - Zirconium/*chemistry
EDAT- 2003/02/06 04:00
MHDA- 2003/07/29 05:00
CRDT- 2003/02/06 04:00
PHST- 2003/02/06 04:00 [pubmed]
PHST- 2003/07/29 05:00 [medline]
PHST- 2003/02/06 04:00 [entrez]
AID - S0021-9673(02)01662-X [pii]
AID - 10.1016/s0021-9673(02)01662-x [doi]
PST - ppublish
SO  - J Chromatogr A. 2003 Jan 3;983(1-2):83-9. doi: 10.1016/s0021-9673(02)01662-x.