PMID- 12570309
OWN - NLM
STAT- MEDLINE
DCOM- 20030306
LR  - 20190702
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 152
IP  - 3
DP  - 2003 Jan 18
TI  - Transmission of porcine reproductive and respiratory syndrome virus by houseflies 
      (Musca domestica).
PG  - 73-6
AB  - Three hundred houseflies were allowed to feed on donor pigs viraemic with porcine 
      reproductive and respiratory syndrome virus (PRRSV) on the fifth, sixth and 
      seventh days after the pigs had been inoculated with the virus. After 60 seconds, 
      the flies' feeding was interrupted, and they were transferred manually to feed to 
      repletion on a naive recipient pig housed in a separate room. To enhance the 
      chance of the flies obtaining the pigs' blood, the back of each pig was scarified 
      with sandpaper until a slight haemorrhage was visible. The PRRSV was transmitted 
      from the donor to the recipient pigs, and PRRSV RNA was detected by reverse 
      transcriptase-PCR from homogenates of the flies. In a second experiment, 210 
      houseflies were allowed to feed to repletion on a PRRSV-infected pig on the sixth 
      day after it had been inoculated, and were then maintained under laboratory 
      conditions. Groups of 30 flies were collected immediately after they had fed and 
      six, 12, 24, 48, 72 and 96 hours later, and were tested for PRRSV. Homogenates of 
      the flies collected up to six hours after feeding were PCR- and pig 
      bioassay-positive, but the others were negative by both tests.
FAU - Otake, S
AU  - Otake S
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St Paul, MN 55108, USA.
FAU - Dee, S A
AU  - Dee SA
FAU - Rossow, K D
AU  - Rossow KD
FAU - Moon, R D
AU  - Moon RD
FAU - Trincado, C
AU  - Trincado C
FAU - Pijoan, C
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - *Houseflies
MH  - *Insect Vectors
MH  - Porcine Reproductive and Respiratory 
      Syndrome/*epidemiology/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/isolation & purification
MH  - RNA, Viral/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Time Factors
EDAT- 2003/02/07 04:00
MHDA- 2003/03/07 04:00
CRDT- 2003/02/07 04:00
PHST- 2003/02/07 04:00 [pubmed]
PHST- 2003/03/07 04:00 [medline]
PHST- 2003/02/07 04:00 [entrez]
AID - 10.1136/vr.152.3.73 [doi]
PST - ppublish
SO  - Vet Rec. 2003 Jan 18;152(3):73-6. doi: 10.1136/vr.152.3.73.