PMID- 12571940
OWN - NLM
STAT- MEDLINE
DCOM- 20031027
LR  - 20111206
IS  - 1000-7423 (Print)
IS  - 1000-7423 (Linking)
VI  - 19
IP  - 3
DP  - 2001
TI  - [Screening and cloning of genes encoding Schistosoma japonicum antigens related 
      to the serum antibodies in Mirotus fortis].
PG  - 153-6
AB  - OBJECTIVE: To understand and identify the molecules related to the natural 
      resistance to Schistosoma japonicum infection in Mirotus fortis. METHODS: Sera 
      from Mirotus fortis without schistosome infection were collected. The S. 
      japonicum adult worm cDNA library was immunologically screened with the sera. The 
      positive recombinants were identified, cloned, sequenced and analysed with 
      software and internet. RESULTS: Seven genes encoding antigens relevant to sera 
      antibodies in Mirotus fortis were cloned and sequenced. These antigens included 
      glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors 
      (SERPIN), 70 kDa heat shock protein (HSP70), 22.6 kDa membrane-associated 
      antigen, paramyosin (Sj97), cytochrome C and cathepsin B. CONCLUSION: Many 
      protein molecules might have been involved in natural resistance to S. japonicum 
      infection in Mirotus fortis. The above 7 kinds of molecules may be identified as 
      new candidates of vaccine against S. japonicum infection.
FAU - Yan, Y T
AU  - Yan YT
AD  - Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, 
      Shanghai, 200025.
FAU - Liu, S X
AU  - Liu SX
FAU - Song, G C
AU  - Song GC
FAU - Xu, Y X
AU  - Xu YX
FAU - He, Y K
AU  - He YK
LA  - chi
GR  - IP50A139461/IP/NCIRD CDC HHS/United States
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - China
TA  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
JT  - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of 
      parasitology & parasitic diseases
JID - 8709992
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (DNA, Helminth)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/blood
MH  - Antigens, Helminth/*genetics
MH  - Arvicolinae/*immunology
MH  - Cloning, Molecular
MH  - DNA, Helminth/genetics
MH  - Gene Library
MH  - Schistosoma japonicum/*genetics/immunology
MH  - Schistosomiasis japonica/*immunology
EDAT- 2003/02/08 04:00
MHDA- 2003/10/28 05:00
CRDT- 2003/02/08 04:00
PHST- 2003/02/08 04:00 [pubmed]
PHST- 2003/10/28 05:00 [medline]
PHST- 2003/02/08 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2001;19(3):153-6.