PMID- 12572683
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20191106
IS  - 0966-0844 (Print)
IS  - 0966-0844 (Linking)
VI  - 16
IP  - 2
DP  - 2003 Jun
TI  - Cadmium stress studies: media development, enrichment, consortia analysis, and 
      environmental relevance.
PG  - 251-61
AB  - The effects of Cadmium (Cd) toxicity on bacterial consortia originating from 
      an-aerobic sewage sludge and cultivated under differing enrichment conditions 
      were studied. Cultures were enriched in minimal media developed specifically for 
      Cd stress studies. At inoculation all Cd was soluble in free ion or chelated 
      form. Electron donors and acceptors were varied to obtain each physiological 
      enrichment type. Adaptation leading to higher levels of Cd resistance of the 
      consortia over time was observed under all physiological conditions. Initial and 
      increased Cd tolerances were consistently greatest in multiphysiological 
      enrichments (MPH). Sulfate reducing (SRB), methanogenic (MET), and fermentative 
      (FRM) enrichments had less tolerance however, the level of tolerance to the Cd 
      varied from one inoculation to the next. The Cd remained soluble as free Cd in 
      MPH and FRM conditions and was precipitated significantly in SRB and moderately 
      in MET conditions. Denaturing gradient gel electrophoresis (DGGE) of polymerase 
      chain reaction (PCR) amplified 16S rRNA of the SRB, MPH, and FRM enrichments were 
      followed over time. The consortia underwent succession under all physiological 
      conditions when compared with the profile of the inoculum. Microbial population 
      diversity decreased as the consortia were subcultured. The effects of chelators 
      in the MPH medium were also evaluated. The addition of chelators transiently 
      decreased toxicity. Effects of MPH medium on the Cd sorption capacity of soil 
      were evaluated. Microbial growth decreased the amount of Cd left in solution.
FAU - Knotek-Smith, Heather M
AU  - Knotek-Smith HM
AD  - Department of Agricultural and Biological Systems Engineering, University of 
      Idaho Moscow, ID, 83844-1052, USA.
FAU - Deobald, Lee A
AU  - Deobald LA
FAU - Ederer, Martina
AU  - Ederer M
FAU - Crawford, Don L
AU  - Crawford DL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Biometals
JT  - Biometals : an international journal on the role of metal ions in biology, 
      biochemistry, and medicine
JID - 9208478
RN  - 0 (Chelating Agents)
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 00BH33GNGH (Cadmium)
SB  - IM
MH  - Bacteria/*drug effects/genetics/*growth & development
MH  - Cadmium/metabolism/*toxicity
MH  - Cell Division/drug effects
MH  - Chelating Agents/pharmacology
MH  - Culture Media/*chemistry
MH  - DNA, Bacterial/analysis
MH  - Drug Resistance, Bacterial
MH  - Hydrogen-Ion Concentration
MH  - Klebsiella/drug effects/genetics/growth & development
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/genetics
MH  - Soil/analysis
MH  - *Soil Microbiology
MH  - Soil Pollutants/metabolism/toxicity
EDAT- 2003/02/08 04:00
MHDA- 2003/12/16 05:00
CRDT- 2003/02/08 04:00
PHST- 2003/02/08 04:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/02/08 04:00 [entrez]
AID - 10.1023/a:1020617013927 [doi]
PST - ppublish
SO  - Biometals. 2003 Jun;16(2):251-61. doi: 10.1023/a:1020617013927.