PMID- 12573473
OWN - NLM
STAT- MEDLINE
DCOM- 20030416
LR  - 20191210
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 122
IP  - 2
DP  - 2003 Jan 30
TI  - Detection of single unit activity from the rat vagus using cluster analysis of 
      principal components.
PG  - 141-7
AB  - In vivo recordings from subdiaphragmatic vagal afferent nerves generally lack the 
      resolution to distinguish single unit activity. Several methods for data 
      acquisition and analysis were combined to produce a high degree of reliability in 
      recording electrophysiological signals from gastrointestinal and hepatic afferent 
      fibers in the rat. Recordings with low noise were achieved by paralysis of the 
      respiratory muscles and by pinning the nerve to a recording platform. Single unit 
      activity was isolated using principal component (PC) analysis and cluster cutting 
      of data in multi-dimensional space (1-3 PCs). Cluster assignments were determined 
      by a semi-automated approach using the k-means algorithm. The accuracy of single 
      unit classification was assessed by checking inter-spike intervals (ISIs) to 
      determine the length of the refractory period, and by cross-correlation analysis 
      to assess whether single units were mistakenly split into more than one cluster. 
      These analyses produced up to four isolated single units from each nerve filament 
      (a bundle of nerve fibers), and typically it was possible to further increase 
      yield by recording from several nerve filaments simultaneously using an array of 
      electrodes.
FAU - Horn, Charles C
AU  - Horn CC
AD  - The Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, 
      USA. horn@monell.org
FAU - Friedman, Mark I
AU  - Friedman MI
LA  - eng
GR  - DK02894/DK/NIDDK NIH HHS/United States
GR  - DK36339/DK/NIDDK NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
SB  - IM
MH  - Action Potentials/*physiology
MH  - Animals
MH  - Cluster Analysis
MH  - Diaphragm/innervation/physiology
MH  - Electrophysiology/*methods
MH  - Male
MH  - Nerve Fibers/*classification/*physiology
MH  - Neurons, Afferent/classification/physiology
MH  - Principal Component Analysis
MH  - Rats
MH  - Rats, Inbred F344
MH  - *Signal Processing, Computer-Assisted
MH  - Statistics as Topic
MH  - Vagus Nerve/*physiology
EDAT- 2003/02/08 04:00
MHDA- 2003/04/17 05:00
CRDT- 2003/02/08 04:00
PHST- 2003/02/08 04:00 [pubmed]
PHST- 2003/04/17 05:00 [medline]
PHST- 2003/02/08 04:00 [entrez]
AID - S0165027002003047 [pii]
AID - 10.1016/s0165-0270(02)00304-7 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2003 Jan 30;122(2):141-7. doi: 10.1016/s0165-0270(02)00304-7.