PMID- 12573502
OWN - NLM
STAT- MEDLINE
DCOM- 20030428
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 91
IP  - 2
DP  - 2003 Feb
TI  - Defective human T-cell lymphotropic virus type I (HTLV-I) provirus in 
      seronegative tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM) 
      patients.
PG  - 231-9
AB  - Infection with human T-cell lymphotropic virus type I (HTLV-I) have been 
      associated with the development of the tropical spastic 
      paraparesis/HTLV-I-associated myelopathy (TSP/HAM). We studied the presence of 
      HTLV-I provirus in peripheral blood mononuclear cells (PBMC) from 72 Chilean 
      patients with progressive spastic paraparesis by polymerase chain reaction: 32 
      seropositive and 40 seronegative cases. We amplified different genomic regions of 
      HTLV-I using primers of 5' ltr, tax, env/tax, pX, pol and env genes. These genes 
      were detected from all seropositive patients. The seronegative patients were 
      negative with 5' ltr, pol, env, and pX primers. However, amplified product of tax 
      and env/tax genes was detected from 16 and four seronegative patients, 
      respectively. Three of them were positive with both genetic regions. The results 
      of this study show that the complete HTLV-I provirus is found in 100% of 
      seropositive cases. In seronegative cases, clinically very similar of 
      seropositive cases, was found only tax gene in 42.5% (17/40) of patients. These 
      results suggest the presence of a defective HTLV-I provirus in some seronegative 
      patients with progressive spastic paraparesis, and suggest a pathogenic role of 
      this truncate provirus for a group of TSP/HAM.
FAU - Ramirez, E
AU  - Ramirez E
AD  - Section of Virology, Public Health Institute of Chile, Avenida Marathon 1000, 
      Santiago, Chile. eramirez@ispch.cl
FAU - Fernandez, J
AU  - Fernandez J
FAU - Cartier, L
AU  - Cartier L
FAU - Villota, C
AU  - Villota C
FAU - Rios, M
AU  - Rios M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - Chile
MH  - DNA, Viral/blood
MH  - Defective Viruses/*genetics/isolation & purification
MH  - Female
MH  - Gene Products, tax/chemistry/genetics
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-I Infections/*virology
MH  - Human T-lymphotropic virus 1/*genetics/isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/*virology
MH  - Proviruses/*genetics/isolation & purification
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/chemistry/genetics
EDAT- 2003/02/08 04:00
MHDA- 2003/04/29 05:00
CRDT- 2003/02/08 04:00
PHST- 2003/02/08 04:00 [pubmed]
PHST- 2003/04/29 05:00 [medline]
PHST- 2003/02/08 04:00 [entrez]
AID - S0168170202002769 [pii]
AID - 10.1016/s0168-1702(02)00276-9 [doi]
PST - ppublish
SO  - Virus Res. 2003 Feb;91(2):231-9. doi: 10.1016/s0168-1702(02)00276-9.