PMID- 12573914
OWN - NLM
STAT- MEDLINE
DCOM- 20030513
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 111
IP  - 2
DP  - 2003 Feb
TI  - Identification of antigenic epitopes on human allergens: studies with HLA 
      transgenic mice.
PG  - 245-50
AB  - Environmental factors play an important role in the rise and manifestation of 
      allergic conditions in genetically predisposed subjects. Increased exposure to 
      indoor/outdoor allergens is a significant factor in the development of allergic 
      sensitization and asthma. Recently, strong relationships between the immune 
      response to several highly purified allergens and specific human leukocyte 
      antigen (HLA)-DQ and -DR haplotypes have been reported. The major antigens from 
      clinically important allergens have been cloned and sequenced. However, whether 
      innate structural features of major allergens or peculiar immune recognition of 
      these molecules contribute to the overly robust immune responses is not known. We 
      generated and used transgenic (tg) mice expressing single HLA class II 
      transgene(s) to characterize the allergen epitopes presented by particular HLA 
      class II molecules. Next, we generated in vivo models for asthma in the HLA tg 
      mice by intranasal challenge with allergenic extracts. Furthermore, we used a 
      single epitope to induce an allergic lung inflammation. Our system offers a 
      sophisticated technique for systematically identifying the genetic (individual 
      human class II) and antigenic (individual allergenic epitopes) basis of asthma 
      sensitivity and has important implications for new treatment strategies.
FAU - Chapoval, Svetlana P
AU  - Chapoval SP
AD  - Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - David, Chella S
AU  - David CS
LA  - eng
GR  - AI 14764/AI/NIAID NIH HHS/United States
GR  - CA09127/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Asthma/etiology/*genetics/immunology
MH  - Disease Models, Animal
MH  - Epitopes
MH  - *Genetic Predisposition to Disease
MH  - HLA-DQ Antigens/*genetics/immunology
MH  - HLA-DR Antigens/*genetics/immunology
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
PMC - PMC1241359
EDAT- 2003/02/08 04:00
MHDA- 2003/05/14 05:00
PMCR- 2003/02/01
CRDT- 2003/02/08 04:00
PHST- 2003/02/08 04:00 [pubmed]
PHST- 2003/05/14 05:00 [medline]
PHST- 2003/02/08 04:00 [entrez]
PHST- 2003/02/01 00:00 [pmc-release]
AID - 10.1289/ehp.5706 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2003 Feb;111(2):245-50. doi: 10.1289/ehp.5706.