PMID- 12575182
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20171116
IS  - 0257-7712 (Print)
IS  - 0257-7712 (Linking)
VI  - 33
IP  - 2
DP  - 2002 Apr
TI  - [Antisense inhibition of gene expression in human dendritic cells by peptide 
      nucleic acid against CD86].
PG  - 192-5
AB  - OBJECTIVE: Dendritic cells (DCs) are the most potent antigen presenting cells 
      (APCs) of the immune system. We intent to block the expression of CD86 in DCs 
      using antisense peptide nucleic acids (PNA), a novel synthetic structural DNA 
      mimic, and interrupt the second signal transmission so that a suppression of 
      corresponding T cell function can be achieved. METHODS: Human DCs grown up from 
      peripheral blood monocytes in GM-CSF and IL-4 were collected. We investigated 
      antisense PNA internalization with laser scan confocal microscope (LSCM). 
      Fluorescence immunocytochemistry, flow cytometry and RT-PCR were used to 
      determine the expression of CD86 protein and mRNA in DCs. RESULTS: LSCM proved 
      that cultured immature DCs could internalized PNA efficiently, according to the 
      specific internalization property of the immature DCs. Antisense PNA DC exhibited 
      striking reductions in cell surface staining for CD86, but not MHC class II, and 
      were poor stimulators of T cell proliferation. RT-PCR found that PNA depressed 
      the amounts of CD86 mRNA in DCs. CONCLUSION: Antisense PNA against CD86 could 
      inhibit the expression of CD86 mRNA and protein in DCs. The blockade of B7/CD28 
      pathway may increase the potential of costimulatory molecule-deficient antisense 
      PNA DCs of donor origin to induce long-lasting allograft survival.
FAU - Feng, Gang
AU  - Feng G
AD  - Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan 
      University, Chengdu 610041, China.
FAU - Li, Shengfu
AU  - Li S
FAU - Li, Youping
AU  - Li Y
FAU - Bu, Hong
AU  - Bu H
FAU - Yang, Yuru
AU  - Yang Y
FAU - Lu, Yiping
AU  - Lu Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Hua Xi Yi Ke Da Xue Xue Bao
JT  - Hua xi yi ke da xue xue bao = Journal of West China University of Medical 
      Sciences = Huaxi yike daxue xuebao
JID - 8609552
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (DNA, Antisense)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigens, CD/*biosynthesis/genetics
MH  - B7-2 Antigen
MH  - Cells, Cultured
MH  - DNA, Antisense/pharmacology
MH  - Dendritic Cells/*metabolism
MH  - Humans
MH  - Membrane Glycoproteins/*biosynthesis/genetics
MH  - Peptide Nucleic Acids/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2003/02/11 04:00
MHDA- 2003/05/31 05:00
CRDT- 2003/02/11 04:00
PHST- 2003/02/11 04:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/02/11 04:00 [entrez]
PST - ppublish
SO  - Hua Xi Yi Ke Da Xue Xue Bao. 2002 Apr;33(2):192-5.