PMID- 12576588
OWN - NLM
STAT- MEDLINE
DCOM- 20030417
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 149
IP  - Pt 1
DP  - 2003 Jan
TI  - Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th 
      century Hungarians.
PG  - 143-51
AB  - The naturally mummified remains of a mother and two daughters found in an 18th 
      century Hungarian crypt were analysed, using multiple molecular genetic 
      techniques to examine the epidemiology and evolution of tuberculosis. DNA was 
      amplified from a number of targets on the Mycobacterium tuberculosis genome, 
      including DNA from IS6110, gyrA, katG codon 463, oxyR, dnaA-dnaN, mtp40, plcD and 
      the direct repeat (DR) region. The strains present in the mummified remains were 
      identified as M. tuberculosis and not Mycobacterium bovis, from katG and gyrA 
      genotyping, PCR from the oxyR and mtp40 loci, and spoligotyping. Spoligotyping 
      divided the samples into two strain types, and screening for a deletion in the 
      MT1801-plcD region initially divided the strains into three types. Further 
      investigation showed, however, that an apparent deletion was due to poor DNA 
      preservation. By comparing the effect of PCR target size on the yield of 
      amplicon, a clear difference was shown between 18th century and modern M. 
      tuberculosis DNA. A two-centre system was used to confirm the findings of this 
      study, which clearly demonstrate the value of using molecular genetic techniques 
      to study historical cases of tuberculosis and the care required in drawing 
      conclusions. The genotyping and spoligotyping results are consistent with the 
      most recent theory of the evolution and spread of the modern tuberculosis 
      epidemic.
FAU - Fletcher, Helen A
AU  - Fletcher HA
AD  - Department of Medical Microbiology, University College London, London W1T 4JF, 
      UK.
FAU - Donoghue, Helen D
AU  - Donoghue HD
FAU - Taylor, G Michael
AU  - Taylor GM
FAU - van der Zanden, Adri G M
AU  - van der Zanden AG
FAU - Spigelman, Mark
AU  - Spigelman M
LA  - eng
PT  - Historical Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bacterial Proteins/genetics/metabolism
MH  - DNA Transposable Elements/genetics
MH  - DNA, Bacterial/*analysis/genetics
MH  - Family
MH  - Female
MH  - Genotype
MH  - History, 18th Century
MH  - Humans
MH  - Hungary/epidemiology
MH  - Middle Aged
MH  - Molecular Epidemiology
MH  - Mothers
MH  - Mycobacterium tuberculosis/*classification/*genetics
MH  - Nuclear Family
MH  - Oligonucleotides/analysis
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Tuberculosis/epidemiology/*history/microbiology
EDAT- 2003/02/11 04:00
MHDA- 2003/04/18 05:00
CRDT- 2003/02/11 04:00
PHST- 2003/02/11 04:00 [pubmed]
PHST- 2003/04/18 05:00 [medline]
PHST- 2003/02/11 04:00 [entrez]
AID - 10.1099/mic.0.25961-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2003 Jan;149(Pt 1):143-51. doi: 10.1099/mic.0.25961-0.