PMID- 12578173 OWN - NLM STAT- MEDLINE DCOM- 20030502 LR - 20131121 IS - 0273-1223 (Print) IS - 0273-1223 (Linking) VI - 47 IP - 1 DP - 2003 TI - Effect of dissolved oxygen concentration on the biofilm and in situ analysis by fluorescence in situ hybridization (FISH) and microelectrodes. PG - 49-57 AB - A better understanding of microbiology and ecology of nitrifying bacteria in inner biofilms is an important part of improving process performance and control. Microelectrodes and fluorescent in situ hybridization (FISH) in biofilm research have been used to investigate the spatial distributions of various microbial activities in biofilms and have led to new experimental findings as well as modifications of the homogeneous assumptions in the biofilm kinetic models. The objective of this study is to try the combination of two methods, both FISH and microelectrode measurements, and to provide reliable and in situ information on nitrifying bacterial activity in biofilms. The characteristics of biofilm developed on tygon slides were different according to the change of dissolved oxygen (DO). When the DO increased from 2 to 10 mg DO/L, the rate of the biofilm thickness increased and its dry density changed from 50-70 to 25-90 mg/cm3. Ammonia oxidizing bacteria were not uniformly distributed in biofilm, and were found at the deeper layer where oxygen is depleted, they were detected primarily in the upper and middle layers of the biofilm. FAU - Jang, A AU - Jang A AD - Department of Environ. Sci. and Eng., Kwangju Institute of Science and Technology (K-JIST), 1 Oryongdong, Puk-gu, Kwangju 500-712, Korea. FAU - Bishop, P L AU - Bishop PL FAU - Okabe, S AU - Okabe S FAU - Lee, S G AU - Lee SG FAU - Kim, I S AU - Kim IS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Water Sci Technol JT - Water science and technology : a journal of the International Association on Water Pollution Research JID - 9879497 RN - N762921K75 (Nitrogen) RN - S88TT14065 (Oxygen) SB - IM MH - Bacteria MH - *Biofilms MH - Electrodes MH - Environmental Monitoring MH - In Situ Hybridization, Fluorescence MH - Kinetics MH - Nitrogen/*metabolism MH - Oxygen/*analysis MH - Solubility MH - Waste Disposal, Fluid EDAT- 2003/02/13 04:00 MHDA- 2003/05/03 05:00 CRDT- 2003/02/13 04:00 PHST- 2003/02/13 04:00 [pubmed] PHST- 2003/05/03 05:00 [medline] PHST- 2003/02/13 04:00 [entrez] PST - ppublish SO - Water Sci Technol. 2003;47(1):49-57.