PMID- 12578975
OWN - NLM
STAT- MEDLINE
DCOM- 20030401
LR  - 20231105
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 100
IP  - 4
DP  - 2003 Feb 18
TI  - Insights into glutamate transport regulation in human astrocytes: cloning of the 
      promoter for excitatory amino acid transporter 2 (EAAT2).
PG  - 1955-60
AB  - Glutamate transport is central to neurotransmitter functions in the brain. 
      Impaired glutamate transport induces neurotoxicity associated with numerous 
      pathological processes, including stroke/ischemia, temporal lobe epilepsy, 
      Alzheimer's disease, amyotrophic lateral sclerosis, Huntington's disease, 
      HIV-1-associated dementia, and growth of malignant gliomas. Excitatory amino acid 
      transporter-2 (EAAT2) is a major glutamate transporter in the brain expressed 
      primarily in astrocytes. We presently describe the cloning and characterization 
      of the human EAAT2 promoter, demonstrating elevated expression in astrocytes. 
      Regulators of EAAT2 transport, both positive and negative, alter EAAT2 
      transcription, promoter activity, mRNA, and protein. These findings imply that 
      transcriptional processes can regulate EAAT2 expression. Moreover, they raise the 
      intriguing possibility that the EAAT2 promoter may be useful for targeting gene 
      expression in the brain and for identifying molecules capable of modulating 
      glutamate transport that could potentially inhibit, ameliorate, or prevent 
      various neurodegenerative diseases.
FAU - Su, Zao-zhong
AU  - Su ZZ
AD  - Department of Pathology, Herbert Irving Comprehensive Cancer Center, College of 
      Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
FAU - Leszczyniecka, Magdalena
AU  - Leszczyniecka M
FAU - Kang, Dong-chul
AU  - Kang DC
FAU - Sarkar, Devanand
AU  - Sarkar D
FAU - Chao, Wei
AU  - Chao W
FAU - Volsky, David J
AU  - Volsky DJ
FAU - Fisher, Paul B
AU  - Fisher PB
LA  - eng
SI  - GENBANK/AF510107
GR  - P01 NS031492/NS/NINDS NIH HHS/United States
GR  - NS31492/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030210
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (RNA, Messenger)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Astrocytes/*metabolism
MH  - Base Sequence
MH  - Biological Transport
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Excitatory Amino Acid Transporter 2/*genetics
MH  - Glutamic Acid/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Transcription, Genetic
PMC - PMC149940
EDAT- 2003/02/13 04:00
MHDA- 2003/04/02 05:00
PMCR- 2003/08/18
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/04/02 05:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
PHST- 2003/08/18 00:00 [pmc-release]
AID - 0136555100 [pii]
AID - 6555 [pii]
AID - 10.1073/pnas.0136555100 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1955-60. doi: 
      10.1073/pnas.0136555100. Epub 2003 Feb 10.