PMID- 12579802
OWN - NLM
STAT- MEDLINE
DCOM- 20031208
LR  - 20061115
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 37
IP  - 6
DP  - 2002 Jun
TI  - [Analysis of the diterpenoids in the extract of Pteris semipinnata L by 
      HPLC-APCI-MS].
PG  - 444-6
AB  - AIM: To establish an accurate and reliable method for quantitative analysis of 
      the diterpenoids in Pteris semipinnata L. METHODS: A quadruple mass spectrometer 
      coupled with atmospheric pressure chemical ionization interface was employed as a 
      detector for HPLC. As to MS detector, selective ion monitoring (SIM) scan mode 
      was used. For ent-11 alpha-hydroxy-15-oxo-kaur-16-en-19-olic acid (5F) and ent-11 
      alpha-hydroxy-15-oxo-kaur-16(R) methyl-19-olic acid (4F), the majority of the 
      diterpenoids in Pteris semipinnata L, the [M-H]-1 ion were observed, and the 
      [M-H2O-H]-1 ion could be observed from the collision-induced dissociation 
      spectua. [M-H]-1 was selected as the SIM ion in quantification, the mobile phase 
      and the MS conditions were optimized. The mobile phase of HPLC was 30% CH3CN-70% 
      2 mmol.L-1 NH4Ac, analytical column was Diamonsil ODS (4.6 mm x 150 mm), flow 
      rate 1.0 mL.min-1, inject volume 5 microL. The area of ion flow peak were used 
      for quantitative determination. As an example of its application, this method was 
      used to determine the content of 5F as an antitumor diterpenoid in Pteris 
      semipinnata L. RESULTS: The content of 5F accounted 1.18 mg.g-1 in Pteris 
      semipinnata L sample. For 5F, RT is about 4.3 min, the standard curve showed good 
      linearity over the range of 0.05-2.5 micrograms, gamma = 0.9998 (n = 5); the 
      recovery was 97.8% (n = 5); the limit of detection was 0.4 ng (inject 5 microL). 
      CONCLUSION: This method is highly sensitive, accurate and fast, which can be 
      applied to study the antitumor drug of diterpenoids in Pteris semipinnata L and 
      to establish the raw herb standard.
FAU - Deng, Yi-feng
AU  - Deng YF
AD  - Guangdong Medical College, Key Laboratory of Natural Drugs Research and 
      Development of Guangdong, Zhanjiang 524023, China. dengfy@gdmc.edu.cn
FAU - Liang, Nian-ci
AU  - Liang NC
FAU - Liang, Tong
AU  - Liang T
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (11-hydroxy-15-oxokaur-16-en-19-olic acid)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Diterpenes)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*analysis/chemistry/pharmacology
MH  - Chromatography, High Pressure Liquid/methods
MH  - Diterpenes/*analysis/chemistry/pharmacology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Molecular Structure
MH  - Plants, Medicinal/*chemistry
MH  - Pteris/*chemistry
MH  - Quality Control
EDAT- 2003/02/13 04:00
MHDA- 2003/12/10 05:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/12/10 05:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
PST - ppublish
SO  - Yao Xue Xue Bao. 2002 Jun;37(6):444-6.