PMID- 12580811
OWN - NLM
STAT- MEDLINE
DCOM- 20030611
LR  - 20190822
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 1
DP  - 2003 Jan
TI  - HLA-DR-dependent increased mannan-induced lymphoproliferative response in atopic 
      eczema dermatitis syndrome.
PG  - 72-7
AB  - BACKGROUND: In yeast-sensitive atopic eczema dermatitis syndrome (AEDS), yeast 
      mannan induces highly elevated specific IgE levels and lymphoproliferative 
      responses. In healthy individuals the involvement of both human leukocyte antigen 
      (HLA)-dependent T-cell activation and non-HLA-dependent activation, e.g. by 
      crosslinking of the cell surface mannose receptors, has been suggested. In the 
      present study the HLA dependence and the role of crosslinking in the 
      lymphoproliferative response to mannan in AEDS has been analyzed. METHODS: Twenty 
      patients with AEDS and 12 controls with no history of allergic diseases were 
      included in the study. Mannan from Candida albicans was prepared according to the 
      Cetavlon method. Following isolation using Ficoll-Hypaque, peripheral blood 
      mononuclear cells (PBMC) were incubated with the mannan preparation in the 
      absence and presence of different concentrations of neutralizing anti-HLA 
      antibodies and alpha-methylmannoside for 6 days and proliferative responses were 
      measured by 3H-thymidine incorporation and scintilloradiography. RESULTS: In AEDS 
      patients with elevated mannan-specific serum IgE, the C. albicans mannan induced 
      lymphoproliferation. Mannan-induced lymphoproliferative responses could be 
      inhibited, dose-dependently, by neutralizing anti-HLA-DR, but not anti-HLA-DQ 
      antibodies in AEDS patients and healthy controls. The addition of 
      alpha-methylmannoside, that blocks binding to mannose receptors, inhibited 
      lymphoproliferative responses in a dose-dependent way by 50% only in healthy 
      controls, but not in AEDS patients. Levels of inhibition of the proliferation by 
      alpha-methylmannoside correlated inversely with the yeast- and mannan-specific 
      IgE levels. CONCLUSIONS: These results show that in healthy subjects yeast mannan 
      activates lymphocytes both in an HLA-DR-dependent manner and as a result of 
      direct crosslinking of the cell surface. However, in AEDS the elevated 
      lymphoproliferative response is HLA-DR-dependent, although only a slight 
      proportion of this response results from direct crosslinking.
FAU - Savolainen, J
AU  - Savolainen J
AD  - INSERM U454, Hopital Arnaud de Villeneuve, Montpellier, France.
FAU - Kosonen, J
AU  - Kosonen J
FAU - Kortekangas-Savolainen, O
AU  - Kortekangas-Savolainen O
FAU - Yssel, H
AU  - Yssel H
FAU - Bousquet, J
AU  - Bousquet J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Mannans)
RN  - 0 (Methylmannosides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 51023-63-3 (methylmannoside)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/drug effects/immunology
MH  - Candida albicans/immunology
MH  - Dermatitis, Atopic/blood/*immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Finland
MH  - HLA-DQ Antigens/*immunology
MH  - Humans
MH  - Immunity, Cellular/drug effects/immunology
MH  - Immunoglobulin E/blood/drug effects/immunology
MH  - Lymphocyte Activation/drug effects/*immunology
MH  - Male
MH  - Mannans/*immunology
MH  - Methylmannosides/administration & dosage/antagonists & inhibitors
MH  - Statistics as Topic
MH  - Syndrome
EDAT- 2003/02/13 04:00
MHDA- 2003/06/12 05:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/06/12 05:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - 2s3707 [pii]
AID - 10.1034/j.1398-9995.2003.23707.x [doi]
PST - ppublish
SO  - Allergy. 2003 Jan;58(1):72-7. doi: 10.1034/j.1398-9995.2003.23707.x.