PMID- 12580962
OWN - NLM
STAT- MEDLINE
DCOM- 20030331
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 120
IP  - 3
DP  - 2003 Feb
TI  - Detection of human herpesvirus-8 in peripheral blood mononuclear cells from adult 
      Japanese patients with multicentric Castleman's disease.
PG  - 471-7
AB  - Human herpesvirus-8 (HHV-8) encodes viral homologues of cellular genes, including 
      viral interleukin 6 (vIL-6), which induces endogenous human IL-6 (hIL-6) 
      secretion. Unregulated overproduction of hIL-6 in lymph nodes (LN) is thought to 
      be responsible for the systemic manifestations of multicentric Castleman's 
      disease (MCD). In the present study, we assessed the presence of HHV-8 and 
      HHV-8-encoded viral homologues in LN and peripheral blood mononuclear cells 
      (PBMC) from adult Japanese patients with MCD. HHV-8 DNA was amplified by nested 
      polymerase chain reaction (PCR) and was detected in LN from 13 out of 16 MCD 
      patients (81%). HHV-8 DNA was also detected in PBMC from six out of seven 
      patients (86%) whose LN were positive for HHV-8 DNA. Because mRNA could not be 
      successfully extracted from LN sections that were either formalin-fixed or 
      embedded in paraffin, we examined the expression of mRNA for HHV-8-encoded viral 
      homologues, such as vIL-6, vBCL-2, vCyclin-D and viral G-protein-coupled receptor 
      (vGPCR) by nested reverse transcription (RT)-PCR in PBMC from 10 MCD patients. 
      However, mRNA of these HHV-8-encoded viral homologues was not detected in any 
      patients tested. Although our results do not indicate a role for HHV-8-encoded 
      viral homologues in the pathogenesis of MCD, they do suggest that HHV-8 infection 
      may be associated with MCD in adult Japanese patients.
FAU - Yamasaki, Satoshi
AU  - Yamasaki S
AD  - Medicine and Biosystemic Science, Kyushu University Graduate School of Medical 
      Sciences, Fukuoka, Japan. yamasaki@1nai.med.kyushu-u.ac.jp
FAU - Iino, Tadafumi
AU  - Iino T
FAU - Nakamura, Minoru
AU  - Nakamura M
FAU - Henzan, Hideho
AU  - Henzan H
FAU - Ohshima, Koichi
AU  - Ohshima K
FAU - Kikuchi, Masahiro
AU  - Kikuchi M
FAU - Otsuka, Teruhisa
AU  - Otsuka T
FAU - Harada, Mine
AU  - Harada M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Castleman Disease/*virology
MH  - DNA, Viral/analysis
MH  - Female
MH  - Herpesviridae Infections/*complications
MH  - Herpesvirus 8, Human/*isolation & purification
MH  - Humans
MH  - Leukocytes, Mononuclear/*virology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
EDAT- 2003/02/13 04:00
MHDA- 2003/04/01 05:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/04/01 05:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - 4120 [pii]
AID - 10.1046/j.1365-2141.2003.04120.x [doi]
PST - ppublish
SO  - Br J Haematol. 2003 Feb;120(3):471-7. doi: 10.1046/j.1365-2141.2003.04120.x.