PMID- 12581863
OWN - NLM
STAT- MEDLINE
DCOM- 20030516
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1593
IP  - 2-3
DP  - 2003 Feb 17
TI  - Role of calcium in phototoxicity of 2-butylamino-2-demethoxy-hypocrellin A to 
      human gastric cancer MGC-803 cells.
PG  - 191-200
AB  - After incubation with 2-butylamino-2-demethoxy-hypocrellin A (2-BA-2-DMHA), 
      photodynamically induced change in the cytoplasmic free calcium concentration 
      ([Ca(2+)](i)) and its effect on cell damage were investigated in human gastric 
      cancer (MGC-803). Fluorescence spectrophotometry measurement indicated that the 
      photosensitization of MGC-803 by 2-BA-2-DMHA caused an increase in intracellular 
      calcium [Ca(2+)](i), and this increase in [Ca(2+)](i) showed a dependence on the 
      concentration of 2-BA-2-DMHA, light dose and extracellular [Ca(2+)](e). This 
      phenomenon of intracellular calcium accumulation was further confirmed by using 
      laser scanning confocal microscopy (LSCM). Furthermore, the results from MTT 
      assay and flow cytometry analysis suggested that chelation of extracellular 
      calcium by EGTA or intracellular calcium by BAPTA could inhibit photodynamically 
      induced cell killing, while increase of [Ca(2+)](i) by thapsigargin (TG), a 
      highly specific inhibitor of the Ca(2+)-ATPase, or by A23187, a calcium ionophore 
      could enhance this action. Meanwhile, the nucleus morphology was also 
      investigated by fluorescence microscopy. The results indicated that the increase 
      in intracellular Ca(2+) concentration was responsible for 2-BA-2-DMHA 
      photodynamically induced damage to MGC-803.
FAU - Zhou, Zhixiang
AU  - Zhou Z
AD  - Center for Molecular Biology, Institute of Biophysics, Chinese Academy of 
      Sciences, 15 Datun Road, Chaoyang District, 100101, Beijing, PR China.
FAU - Yang, Hongying
AU  - Yang H
FAU - Zhang, Zhiyi
AU  - Zhang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (2-butylamino-2-demethoxy-hypocrellin A)
RN  - 0 (Chelating Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Ionophores)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Quinones)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 5QD5427UN7 (Perylene)
RN  - 67526-95-8 (Thapsigargin)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Calcimycin
MH  - Calcium/analysis/antagonists & inhibitors/*metabolism
MH  - Cell Survival/drug effects
MH  - Chelating Agents
MH  - Egtazic Acid/*analogs & derivatives
MH  - Enzyme Inhibitors
MH  - Fluorescent Dyes
MH  - Fura-2
MH  - Humans
MH  - Ionophores
MH  - Light
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Perylene/*analogs & derivatives/*pharmacology
MH  - Photochemotherapy
MH  - Photosensitizing Agents/*pharmacology
MH  - Quinones/*pharmacology
MH  - Stomach Neoplasms/*drug therapy
MH  - Thapsigargin
MH  - Tumor Cells, Cultured
EDAT- 2003/02/13 04:00
MHDA- 2003/05/17 05:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/05/17 05:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - S0167488902003865 [pii]
AID - 10.1016/s0167-4889(02)00386-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2003 Feb 17;1593(2-3):191-200. doi: 
      10.1016/s0167-4889(02)00386-5.