PMID- 12581898
OWN - NLM
STAT- MEDLINE
DCOM- 20030307
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 141
IP  - 1
DP  - 2003 Feb
TI  - Fluorescence in situ hybridization for detecting TP16 MTS1/CDK41 gene deletions 
      in squamous cell carcinoma of the head and neck.
PG  - 49-55
AB  - We have previously shown TP16 MTS1/CDK41 gene deletion in more than 50% of a 
      cohort of squamous cell carcinoma of the head and neck (SCCHN) patients using 
      polymerase chain reaction (PCR). We have performed fluorescence in situ 
      hybridization (FISH) on paraffin-embedded SCCHN specimens from the same cohort to 
      identify the deletion of TP16 MTS1/CDK41CDK41gene. Twenty normal and 19 SCCHN 
      specimens were studied. An alpha-satellite DNA probe specific for chromosome 9 
      and a cosmid probe for the TP16 MTS1/CDK41CDK41gene were used. Of the 19 tumors 
      examined by FISH, 6 had homozygous deletions, 7 were hemizygously deleted, and 
      the remaining 6 showed no evidence of deletion of the TP16 MTS1/CDK41 gene. None 
      of the normal specimens showed TP16 gene deletion. Data obtained from FISH highly 
      correlated with the PCR results for the identification of TP16 MTS1/CDK41 gene 
      deletions. Patients with deletion of the TP16 MTS1/CDK41 gene show a greater 
      tendency toward the development of recurrence and metastasis.
FAU - Namazie, Ali
AU  - Namazie A
AD  - Division of Head and Neck Surgery, UCLA School of Medicine, Los Angeles, CA, USA.
FAU - Alavi, Sassan
AU  - Alavi S
FAU - Aghamohammadi, Neema
AU  - Aghamohammadi N
FAU - Aghamohammadi, Mazda
AU  - Aghamohammadi M
FAU - Calcaterra, Thomas C
AU  - Calcaterra TC
FAU - Wang, Marilene B
AU  - Wang MB
FAU - Srivatsan, Eri S
AU  - Srivatsan ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Female
MH  - *Gene Deletion
MH  - *Genes, p16
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Recurrence, Local
MH  - Neoplasms, Squamous Cell/*genetics/pathology
MH  - Prognosis
MH  - Risk Factors
EDAT- 2003/02/13 04:00
MHDA- 2003/03/08 04:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/03/08 04:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - S0165460802006581 [pii]
AID - 10.1016/s0165-4608(02)00658-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Feb;141(1):49-55. doi: 
      10.1016/s0165-4608(02)00658-1.