PMID- 12581899
OWN - NLM
STAT- MEDLINE
DCOM- 20030307
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 141
IP  - 1
DP  - 2003 Feb
TI  - A novel tumorigenic human prostate epithelial cell line (M2205): molecular 
      cytogenetic characterization demonstrates C-MYC amplification and jumping 
      translocations.
PG  - 56-64
AB  - The paucity of cell lines from early-stage prostate cancer tumors has hindered 
      the recognition of genetic and cellular changes that are associated with the 
      acquisition of tumorigenesis. We describe the chromosomal complement of a novel 
      tumorigenic prostate epithelial cell subline, called M2205, that acquired only 
      three new, consistent chromosomal changes (from those present in the SV40T 
      antigen immortalized parental cell line, P69SV40TAg) when it attained 
      tumor-forming potential. The consistent changes, which were fully characterized 
      using GTG-banding, CBG-banding, silver staining, fluorescence in situ 
      hybridization (FISH), comparative genomic hybridization (CGH), and spectral 
      karyotyping (SKY), involved segmental jumping translocations and resulted in 
      gains in the copy number of genes located on the distal long arm of chromosome 8 
      (8q22 to 8q24.3), including c-myc. Furthermore, the jumping translocations also 
      resulted in ribosomal genes being present in multiple, tandem copies next to the 
      chromatin from 8q. Given the relatively small number of cytogenetic changes 
      present, this subline provides a means for better understanding the cellular 
      changes associated with the acquired chromosomal imbalances. Further studies of 
      this subline could also provide insight as to the mechanism or mechanisms leading 
      to the formation of jumping translocations, as well as potential position effects 
      resulting from the relocation of ribosomal genes next to other cellular genes or 
      oncogenes.
FAU - Jackson-Cook, Colleen
AU  - Jackson-Cook C
AD  - Department of Human Genetics, Medical College of Virginia Campus of Virginia 
      Commonwealth University, Richmond, VA 23298-0033, USA. jacksonc@hsc.vcu.edu
FAU - Zou, Ying
AU  - Zou Y
FAU - Turner, Kristi
AU  - Turner K
FAU - Astbury, Caroline
AU  - Astbury C
FAU - Ware, Joy
AU  - Ware J
LA  - eng
GR  - R01 DK052683/DK/NIDDK NIH HHS/United States
GR  - R01CA58126/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Chromosomes, Human/genetics
MH  - Epithelial Cells/*pathology
MH  - Gene Amplification/*genetics
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2003/02/13 04:00
MHDA- 2003/03/08 04:00
CRDT- 2003/02/13 04:00
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/03/08 04:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - S0165460802006659 [pii]
AID - 10.1016/s0165-4608(02)00665-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Feb;141(1):56-64. doi: 
      10.1016/s0165-4608(02)00665-9.