PMID- 12582496 OWN - NLM STAT- Publisher LR - 20191120 IS - 1432-2242 (Electronic) IS - 0040-5752 (Linking) VI - 105 IP - 4 DP - 2002 Sep TI - Chromosome identification and comparative karyotypic analyses of four Pinus species. PG - 491-497 AB - Chromosomal landmarks in four Pinus species: P. densiflora, P. thunbergii, P. sylvestris, and P. nigra were identified by fluorescence in situ hybridization (FISH) using hapten- or fluorochrome-labeled probes for the plant telomere repeat, centromeric repeat ( PCSR), and rDNA. FISH landmarks were located at the interstitial and proximal regions of chromosomes and allowed us to identify nearly all of the homologous chromosomes in each species. A comparative analysis of the FISH karyotypes among the four species showed that the interstitial FISH signals obtained by hybridization with the telomere and rDNA sequences were stable and could be used to identify homologous chromosomes among species. The identification of homologous chromosomes among species facilitated a detailed comparative karyotype analysis. The results suggest that the degree of chromosomal differentiation among the four Pinus species is very low and that the proximal regions vary in their DNA sequences. The similarities and differences among FISH karyotypes are discussed in relation to phylogeny. FAU - Hizume, M AU - Hizume M AD - Biological Institute, Faculty of Education, Ehime University, Matsuyama 790-8577, Japan. FAU - Shibata, F AU - Shibata F FAU - Matsusaki, Y AU - Matsusaki Y FAU - Garajova, Z AU - Garajova Z LA - eng PT - Journal Article DEP - 20020620 PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 EDAT- 2003/02/13 04:00 MHDA- 2003/02/13 04:00 CRDT- 2003/02/13 04:00 PHST- 2001/10/23 00:00 [received] PHST- 2001/12/11 00:00 [accepted] PHST- 2003/02/13 04:00 [pubmed] PHST- 2003/02/13 04:00 [medline] PHST- 2003/02/13 04:00 [entrez] AID - 10.1007/s00122-002-0975-4 [doi] PST - ppublish SO - Theor Appl Genet. 2002 Sep;105(4):491-497. doi: 10.1007/s00122-002-0975-4. Epub 2002 Jun 20.