PMID- 12582595
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 104
IP  - 6-7
DP  - 2002 May
TI  - Comparison between genetic and physical maps in Zea mays L. of molecular markers 
      linked to resistance against Diatraea spp.
PG  - 908-915
AB  - In the pachytene stage, chromosomes are maximally extended and can easily be 
      distinguished. Therefore, by applying fluorescence in situ hybridization (FISH) 
      to pachytene chromosomes, it is possible to generate a high-resolution physical 
      map of chromosome 9 in maize. Molecular markers ( umc105a on the short arm of 
      chromosome 9, csu145a on the long arm) were used that flank quantitative trait 
      loci (QTL) for sugarcane borer (SCB) and southwestern corn borer (SWCB) 
      resistance. As reference markers, a centromere-specific probe (CentC) and a 
      knob-specific probe (pZm4-21) were utilized. Two fluorescent dyes with four 
      probes were used to physically position these markers. Signals of repetitive DNA 
      sequences in cosmid probes were suppressed by chromosome in situ suppression 
      (CISS) hybridization. FISH signals were strong and reproducible for all probes. 
      We measured the distances in micrometers for four subchromosomal regions and 
      estimated the corresponding number of base pairs. The physical locations of the 
      markers were compared on mitotic metaphase and pachytene chromosomes to the 
      genetic map of chromosome 9. Genetic analysis positioned the two markers for SCB 
      resistance in a central interval representing approximately 33.7% of the genetic 
      length. However, the physical distance between these probes was determined to 
      encompass about 70% of the physical length of chromosome 9. The two markers were 
      located at distal positions on opposite arms of chromosome 9. Physical maps 
      provide valuable information for gene isolation and understanding recombination.
FAU - Sadder, T
AU  - Sadder T
AD  - Department of Plant Breeding and Biotechnology, Institute of Plant Breeding, Seed 
      Science and Population Genetics, University of Hohenheim, 70593 Stuttgart, 
      Germany.
FAU - Weber, G
AU  - Weber G
LA  - eng
PT  - Journal Article
DEP - 20020327
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
EDAT- 2003/02/13 04:00
MHDA- 2003/02/13 04:00
CRDT- 2003/02/13 04:00
PHST- 2001/02/26 00:00 [received]
PHST- 2001/06/14 00:00 [accepted]
PHST- 2003/02/13 04:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2003/02/13 04:00 [entrez]
AID - 10.1007/s00122-001-0797-9 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2002 May;104(6-7):908-915. doi: 10.1007/s00122-001-0797-9. Epub 
      2002 Mar 27.