PMID- 12586482
OWN - NLM
STAT- MEDLINE
DCOM- 20031030
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 91
IP  - 3-4
DP  - 2003 Feb 10
TI  - Synovial MMP-3 and TIMP-1 levels and their correlation with cytokine expression 
      in canine rheumatoid arthritis.
PG  - 199-204
AB  - The purpose of this study was to investigate whether synovial fluid levels of 
      matrix metalloprotease-3 (MMP-3) and tissue inhibitor of metalloprotease-1 
      (TIMP-1) are specifically elevated in canine rheumatoid arthritis (CRA) compared 
      to osteoarthritic joint disorders and if these markers are correlated with a 
      specific pattern of cytokine mRNA expression. Synovial fluid samples of 17 dogs 
      with CRA were analysed for MMP-3 and TIMP-1 by two canine sandwich ELISA 
      (enzyme-linked immunosorbent assay) systems. The synovial mRNA content of tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), interleukin-6 (IL-6), 
      interleukin-12 (IL-12), transforming growth factor-beta (TGF-beta), and 
      interferon-gamma (IFN-gamma) was determined by RT-PCR (reverse 
      transcription-polymerase chain reaction). Dogs with osteoarthritis (n = 50) 
      caused by anterior cruciate ligament rupture (ACLR) were used as controls. A 
      significant rise of MMP-3 was found in the synovial fluid of joints with CRA that 
      could not be balanced by sufficient amounts of TIMP-1. The 30-fold surplus of 
      MMP-3 over TIMP-1 was strongly correlated with the synovial mRNA content of IL-1, 
      IL-12 and TGF-beta. Our results point to the potential use of the synovial levels 
      of MMP-3 as a marker for RA in dogs.
CI  - Copyright Published by 2003 Elsevier Science B.V.
FAU - Hegemann, Nina
AU  - Hegemann N
AD  - Faculty of Veterinary Medicine, Institute of Immunology and Molecular Biology, 
      Free University of Berlin, Philippstr 13, D-10115 Berlin, Germany.
FAU - Wondimu, Assefa
AU  - Wondimu A
FAU - Ullrich, Kirsten
AU  - Ullrich K
FAU - Schmidt, Michael F G
AU  - Schmidt MF
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/enzymology/genetics/*metabolism/*veterinary
MH  - Biomarkers/analysis
MH  - Cytokines/*genetics
MH  - Dog Diseases/enzymology/genetics/*metabolism
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression Regulation
MH  - Male
MH  - Matrix Metalloproteinase 3/*analysis
MH  - RNA, Messenger/genetics/metabolism
MH  - Synovial Fluid/chemistry/enzymology/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/*analysis
EDAT- 2003/02/15 04:00
MHDA- 2003/10/31 05:00
CRDT- 2003/02/15 04:00
PHST- 2003/02/15 04:00 [pubmed]
PHST- 2003/10/31 05:00 [medline]
PHST- 2003/02/15 04:00 [entrez]
AID - S0165242703000059 [pii]
AID - 10.1016/s0165-2427(03)00005-9 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2003 Feb 10;91(3-4):199-204. doi: 
      10.1016/s0165-2427(03)00005-9.