PMID- 12587677
OWN - NLM
STAT- MEDLINE
DCOM- 20030715
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 27
IP  - 1
DP  - 2003 Jan-Feb
TI  - Direct screening of urine for MDMA and MDA by liquid chromatography-tandem mass 
      spectrometry.
PG  - 15-9
AB  - There is a limit in the number of substances detected by commercially available 
      reagents. It is therefore important to have other, complementary techniques. 
      Liquid chromatography-tandem mass spectrometry (LC-MS-MS) may offer one 
      possibility. An LC-MS-MS procedure based on the detection of positive ions after 
      atmospheric pressure chemical ionization (APCI) was developed for direct 
      measurement of 3,4-methylenedioxymethamphetamine (MDMA) and 
      3,4-methylenedioxyamphetamine in urine. It was compared with Online Amphetamines. 
      The LC-MS-MS methodology showed improved sensitivity (1 00-ng/mL cutoff and 
      specificity with a coefficient of variation of 10%. It was compared to the 
      immunochemical analysis using 1000 clinical patient urine samples. The positive 
      samples were confirmed by gas chromatography-mass spectrometry. The LC-MS-MS 
      method detected almost four times as many samples positive for MDMA compared to 
      the immunochemical method, with no false positives and one false negative. Our 
      study suggests that LC-MS-MS offers an alternative to immunochemical methods in 
      drug of abuse screening.
FAU - Nordgren, Helena K
AU  - Nordgren HK
AD  - Department of Medicine, Division of Clinical Pharmacology, Karolinska Institutet, 
      Stockholm, Sweden. Helena.Nordgren@ks.se
FAU - Beck, Olof
AU  - Beck O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*urine
MH  - Chromatography, Liquid
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Immunochemistry
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Substance Abuse Detection/*methods
EDAT- 2003/02/18 04:00
MHDA- 2003/07/16 05:00
CRDT- 2003/02/18 04:00
PHST- 2003/02/18 04:00 [pubmed]
PHST- 2003/07/16 05:00 [medline]
PHST- 2003/02/18 04:00 [entrez]
AID - 10.1093/jat/27.1.15 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2003 Jan-Feb;27(1):15-9. doi: 10.1093/jat/27.1.15.