PMID- 12589646
OWN - NLM
STAT- MEDLINE
DCOM- 20030311
LR  - 20041117
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 99
IP  - 1
DP  - 2003 Feb 25
TI  - The use of CDKN2A deletion as a diagnostic marker for malignant mesothelioma in 
      body cavity effusions.
PG  - 51-6
AB  - BACKGROUND: The distinction between benign reactive mesothelial cells and 
      malignant mesothelial cells in serous effusions is difficult and has an unusually 
      high false negative rate. Unfortunately, there are no generally accepted markers 
      to distinguish between benign reactive and malignant mesothelial cells. 
      Homozygous deletion of CDKN2A is frequent in mesothelioma (present in > 70% of 
      tumors). Therefore, detection of CDKN2A deletion by fluorescence in situ 
      hybridization (FISH) was evaluated as an ancillary test in the cytologic 
      diagnosis of malignant mesothelioma. METHODS: Dual-color FISH for CDKN2A and 
      chromosome 9 centromere was performed on cytolyt-fixed Thinprep slides from 6 
      cytologically suspicious and 7 cytologically positive effusions (all with 
      histologically confirmed mesothelioma) and in 19 cytologically benign effusions 
      (14 pleural effusions, 3 pericardial effusions, and 2 abdominal fluid specimens). 
      Specimens containing > or = 15 nuclei that lacked signals for CDKN2A but showed 
      at least 1 signal for chromosome 9 centromere were considered positive. In 
      samples with negative cytology, the nuclei of at least 100 mesothelial cells were 
      evaluated; whereas, in specimens with positive or suspicious cytology, counting 
      nuclei was done only if < 15% of nuclei showed homozygous loss of CDKN2A. 
      RESULTS: Homozygous deletion was detected in mesothelial cells in six of seven 
      specimens with positive cytology and in six of six specimens with suspicious 
      cytology. Cytologically, there were numerous tumor cells in a single positive 
      specimen without homozygous deletion. All 19 cytologically negative specimens 
      were negative for CDKN2A deletion. CONCLUSIONS: The detection of homozygous 
      CDKN2A deletion by FISH would have been helpful in confirming a diagnosis of 
      mesothelioma over reactive mesothelial cells in 12 of 13 samples with positive or 
      suspicious cytology. Further studies on larger series of patients with suspicious 
      cytology are needed to evaluate the validity and efficiency of this approach for 
      improving the diagnostic accuracy of effusion cytology.
CI  - Copyright 2003 American Cancer Society.
FAU - Illei, Peter B
AU  - Illei PB
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New 
      York 10021, USA.
FAU - Ladanyi, Marc
AU  - Ladanyi M
FAU - Rusch, Valerie W
AU  - Rusch VW
FAU - Zakowski, Maureen F
AU  - Zakowski MF
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Ascitic Fluid/cytology
MH  - Female
MH  - *Gene Deletion
MH  - *Genes, p16
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesothelioma/*diagnosis/*genetics
MH  - Middle Aged
MH  - Pericardial Effusion/cytology
MH  - Pleural Effusion/cytology
MH  - Sensitivity and Specificity
EDAT- 2003/02/18 04:00
MHDA- 2003/03/12 04:00
CRDT- 2003/02/18 04:00
PHST- 2003/02/18 04:00 [pubmed]
PHST- 2003/03/12 04:00 [medline]
PHST- 2003/02/18 04:00 [entrez]
AID - 10.1002/cncr.10923 [doi]
PST - ppublish
SO  - Cancer. 2003 Feb 25;99(1):51-6. doi: 10.1002/cncr.10923.