PMID- 12589652
OWN - NLM
STAT- MEDLINE
DCOM- 20031014
LR  - 20061115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 64
IP  - 4
DP  - 2003 Apr
TI  - Identification of estrogen-regulated genes in the mouse uterus using a 
      delayed-implantation model.
PG  - 405-13
AB  - Gonadal steroid hormones are known to modulate the implantation of the 
      blastocyst, but how the controlling genetics are regulated remains largely 
      unknown. Using a delayed-implantation model, we examined estrogen-regulated genes 
      (ERGs) in the mouse uterus using the differential-display reverse 
      transcription-polymerase chain reaction (DD RT-PCR). Pregnant mice were 
      ovariectomized and injected daily with progesterone (P, 1 mg/mouse), followed by 
      a single injection of estrogen (E, 200 ng/mouse); 24 or 48 hr later, total RNA 
      was extracted from the uterus. Reverse Northern analysis verified the expression 
      patterns of 36 clones out of thousands of RNA species. Only five clones had mRNA 
      levels that were modified, whereas other mRNAs were unchanged or not detectable. 
      Sequence analysis of these, using the Basic Local Alignment Search Tool (BLAST) 
      service, revealed that four of these clones were novel; one clone, designated 
      ERG10, was found to be the mouse homologue of that deleted in oral cancer DOC-1. 
      DOC-1 mRNA was detected all tissues examined, but only in the uterus and cervix 
      was markedly increased 12 hr after E administration, it returned to basal level 
      by 48 hr. One of the novel genes, designated ERG8, had three different forms of 
      mRNAs and was expressed ubiquitously in all examined tissues. In the uterus, the 
      mRNA level of ERG8 also increased 12 hr after E administration. These results 
      suggest that during the implantation process, E differentially regulates several 
      genes depending on cell type. Uterine-specific induction of newly found genes, 
      such as ERG8 and 10, by E appears to be important for the early implantation 
      process.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Lee, Sukwon
AU  - Lee S
AD  - School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
FAU - Lee, Seung-Ah
AU  - Lee SA
FAU - Shim, Chanseob
AU  - Shim C
FAU - Khang, Inkoo
AU  - Khang I
FAU - Lee, Kyung-Ah
AU  - Lee KA
FAU - Park, Young-Mee
AU  - Park YM
FAU - Kang, Byung-Moon
AU  - Kang BM
FAU - Kim, Kyungjin
AU  - Kim K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Estrogens)
SB  - IM
MH  - Animals
MH  - Estrogens/*metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - Mice
MH  - Organ Specificity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Uterus/*metabolism
EDAT- 2003/02/18 04:00
MHDA- 2003/10/15 05:00
CRDT- 2003/02/18 04:00
PHST- 2003/02/18 04:00 [pubmed]
PHST- 2003/10/15 05:00 [medline]
PHST- 2003/02/18 04:00 [entrez]
AID - 10.1002/mrd.10232 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2003 Apr;64(4):405-13. doi: 10.1002/mrd.10232.