PMID- 12589960
OWN - NLM
STAT- MEDLINE
DCOM- 20030514
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 35
IP  - 1
DP  - 2003 Jan 21
TI  - Response of primed human PBMC to synthetic peptides derived from hepatitis B 
      virus envelope proteins: a search for promiscuous epitopes.
PG  - 77-85
AB  - This investigation was aimed at identifying effective T helper cell epitopes to 
      the hepatitis B virus in humans. A panel of synthetic peptides that represent the 
      hepatitis B virus whole envelope proteins was examined for their capability to 
      stimulate peripheral blood mononuclear cells from human subjects infected with 
      hepatitis B virus naturally. In addition, a large number of subjects were 
      examined and their human leukocyte antigen (HLA) class II allele types were 
      identified to determine whether the helper T cell epitope is specific for a 
      particular HLA allele or 'promiscuous'. The peptides of the amino acid residues 
      52-67, 110-125, 190-205, and 228-243 appeared to be immunogenic, and 
      particularly, the 52-67 residue was the most promiscuous epitope peptide. These 
      results would contribute to the better understanding of the helper T cell 
      responses to the hepatitis B virus and provide a useful way in designing 
      epitope-based vaccines and future therapeutic strategies.
FAU - Doh, Hyounmie
AU  - Doh H
AD  - Division of Molecular Life Sciences and College of Pharmacy, Ewha Womans 
      University, 11 Daehyun-Dong, Seoul 120-750, South Korea.
FAU - Roh, Sujin
AU  - Roh S
FAU - Lee, Kyung Wha
AU  - Lee KW
FAU - Kim, Kilhyoun
AU  - Kim K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (L protein, hepatitis B virus)
RN  - 0 (Peptides)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - HLA-DQ Antigens/genetics
MH  - HLA-DR Antigens/genetics
MH  - Hepatitis B virus/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/chemistry/*immunology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Vaccines, Synthetic
MH  - Viral Envelope Proteins/chemistry/*immunology
EDAT- 2003/02/19 04:00
MHDA- 2003/05/15 05:00
CRDT- 2003/02/19 04:00
PHST- 2003/02/19 04:00 [pubmed]
PHST- 2003/05/15 05:00 [medline]
PHST- 2003/02/19 04:00 [entrez]
AID - S0928824402004613 [pii]
AID - 10.1016/S0928-8244(02)00461-3 [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2003 Jan 21;35(1):77-85. doi: 
      10.1016/S0928-8244(02)00461-3.