PMID- 12590705
OWN - NLM
STAT- MEDLINE
DCOM- 20031106
LR  - 20071114
IS  - 1525-8165 (Print)
IS  - 1525-8165 (Linking)
VI  - 11
IP  - 6
DP  - 2002 Dec
TI  - Hematopoietic stem cell functional failure in interleukin-2-deficient mice.
PG  - 905-12
AB  - The effects of interleukin-2 (IL-2) deficiency on hematopoiesis were tested by 
      measuring cellular compositions in peripheral blood, spleen, thymus, and bone 
      marrow of 3- to 5-month-old gene-targeted Il2 null (Il2(-/-)) mice using the 
      Advia 120 Hematology system and fluorescence-activated cell staining (FACS). 
      Il2(-/-) mice developed hematological failure and autoimmune responses, showing 
      variable but significant degrees of anemia, lymphocytopenia, thrombocytopenia, 
      splenomegaly, thymus involution, and weight loss. Surprisingly, Il2(-/-) mice had 
      normal numbers of bone marrow cells (BMCs) with increased numbers of 
      Lin(-)Kit(+)Sca1(+)CD34(-) and Lin(-)Kit(+)Sca1(+)CD34(+) cells that are normally 
      associated with hematopoietic stem cells (HSCs) and progenitor cells. Day-12 
      colony-forming units-spleen cells were slightly reduced in Il2(-/-) mice. When 
      Il2(-/-) and Il2(+/+) mice were compared for long-term HSC function in vivo in 
      the competitive repopulation assay, BMCs from Il2(-/-) donors had 10- to 20-fold 
      less HSC repopulating ability, which affected both myeloid and lymphoid cell 
      lineages. Thus, HSCs from Il2(-/-) mice can proliferate normally but are 
      functionally defective for reconstituting lethally irradiated recipients.
FAU - Chen, J
AU  - Chen J
AD  - Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 
      20892-1652, USA. chenji@nhlbi.nih.gov
FAU - Astle, C M
AU  - Astle CM
FAU - Harrison, D E
AU  - Harrison DE
LA  - eng
GR  - CA 34196/CA/NCI NIH HHS/United States
GR  - R01 HL 58820/HL/NHLBI NIH HHS/United States
GR  - R01 HL 63230/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Hematother Stem Cell Res
JT  - Journal of hematotherapy & stem cell research
JID - 100892915
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Animals
MH  - Autoimmunity
MH  - Blood Cells/cytology
MH  - Bone Marrow Cells/immunology
MH  - Cell Count
MH  - Flow Cytometry
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/pathology/*physiology
MH  - Immunophenotyping
MH  - Interleukin-2/*deficiency/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Phenotype
MH  - Spleen/cytology
MH  - Thymus Gland/cytology
EDAT- 2003/02/20 04:00
MHDA- 2003/11/07 05:00
CRDT- 2003/02/20 04:00
PHST- 2003/02/20 04:00 [pubmed]
PHST- 2003/11/07 05:00 [medline]
PHST- 2003/02/20 04:00 [entrez]
AID - 10.1089/152581602321080565 [doi]
PST - ppublish
SO  - J Hematother Stem Cell Res. 2002 Dec;11(6):905-12. doi: 
      10.1089/152581602321080565.