PMID- 12590713
OWN - NLM
STAT- MEDLINE
DCOM- 20031106
LR  - 20061115
IS  - 1525-8165 (Print)
IS  - 1525-8165 (Linking)
VI  - 11
IP  - 6
DP  - 2002 Dec
TI  - Human mesenchymal stem cells are not of donor origin in patients with severe 
      aplastic anemia who underwent sex-mismatched allogeneic bone marrow transplant.
PG  - 977-84
AB  - Stromal defects are part of the etiology of severe aplastic anemia (SAA), and 
      hematopoietic engraftment is poor in unrelated and mismatched transplant. 
      Therefore, we wanted to find out whether human mesenchymal stem cells (MSC) are 
      partly of donor origin in patients with SAA years after successful bone marrow 
      transplant (BMT). Three SAA patients 3, 5, and 8 years after BMT 
      (cyclophosphamide, ATG) with bone marrow from an HLA-identical sibling donor of 
      the opposite sex were investigated. MSC were grown from patients' bone marrow 
      aspirates according to Caplan et al. The number of MSC that were isolated from 
      SAA bone marrow post transplant was about 10 times lower than in normal controls. 
      Primary cultures of adherent MSC and passage-one cells were analyzed by 
      dual-color interphase fluorescence in situ hybridization (FISH) analysis using 
      centromere-specific DNA probes for X and Y chromosome. FISH did not show any 
      clear evidence of donor cells in the adherent MSC: In all cases, less than 0.5% 
      of nuclei showed a donor-type signal pattern that is well within assay limits. In 
      a female patient, the absence of male donor cells was confirmed by sensitive and 
      quantitative, Y chromosome-specific TaqMan PCR (QYCS-PCR). In contrast, 
      Ficoll-separated hematopoietic cells from the same aspirates were greater than 
      90% of donor origin, as expected. In SAA, as previously found in patients with 
      lysosomal and peroxisomal storage disease, bone marrow MSC remain host-derived 
      despite successful hematopoietic engraftment years after allogeneic BMT.
FAU - Stute, Norbert
AU  - Stute N
AD  - Bone Marrow Transplant Center, University Clinic Hamburg-Eppendorf, Hamburg, 
      Germany. stute@uke.uni-hamburg.de
FAU - Fehse, Boris
AU  - Fehse B
FAU - Schroder, Jens
AU  - Schroder J
FAU - Arps, Sonke
AU  - Arps S
FAU - Adamietz, Peter
AU  - Adamietz P
FAU - Held, Karsten R
AU  - Held KR
FAU - Zander, Axel R
AU  - Zander AR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hematother Stem Cell Res
JT  - Journal of hematotherapy & stem cell research
JID - 100892915
SB  - IM
MH  - Adult
MH  - Anemia, Aplastic/*pathology/therapy
MH  - Bone Marrow Cells/cytology
MH  - *Bone Marrow Transplantation
MH  - Cell Lineage
MH  - Female
MH  - Follow-Up Studies
MH  - Graft Survival
MH  - Hematopoiesis
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesoderm/*cytology
MH  - Middle Aged
MH  - Stem Cells/*cytology
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2003/02/20 04:00
MHDA- 2003/11/07 05:00
CRDT- 2003/02/20 04:00
PHST- 2003/02/20 04:00 [pubmed]
PHST- 2003/11/07 05:00 [medline]
PHST- 2003/02/20 04:00 [entrez]
AID - 10.1089/152581602321080646 [doi]
PST - ppublish
SO  - J Hematother Stem Cell Res. 2002 Dec;11(6):977-84. doi: 
      10.1089/152581602321080646.