PMID- 12591203
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 93
IP  - 1
DP  - 2003 May 2
TI  - Strain specificity of the immune response of pigs following vaccination with 
      various strains of porcine reproductive and respiratory syndrome virus.
PG  - 13-24
AB  - The primary objective of the study was to determine strain specificity of the 
      immune response of pigs following vaccination with selected strains of porcine 
      reproductive and respiratory syndrome virus (PRRSV). The experimental design 
      included five groups (I through V, six pigs per group) free of antibody for PRRSV 
      at the beginning of the experiment (day 0). On day 0, groups III, IV, and V were 
      vaccinated with attenuated versions of PRRSV strains 8, 9, and 14, respectively. 
      On day 21, the immunity of group II (non-vaccinated/challenged controls) and 
      groups III, IV, and V was challenged by exposing each pig to a composite of the 
      virulent versions of these same three strains. On day 35, all pigs, including 
      non-vaccinated/non-challenged pigs of group I, were necropsied. Lungs and 
      selected lymph nodes were examined for lesions. Serum samples obtained at weekly 
      intervals throughout the study and lung lavage fluids obtained at necropsy were 
      tested for the presence of PRRSV and its strain identity. Before challenge the 
      strain of PRRSV identified in the sera of vaccinated pigs was always that with 
      which the particular pig had been vaccinated (i.e. homologous strain), whereas, 
      with one exception, only heterologous strains were identified after challenge. 
      This apparent strain exclusion as a result of vaccination was statistically 
      significant (P = 0.004). The tendency for heterologous strains to predominate 
      after challenge suggests that a pig's immune response to PRRSV has some degree of 
      strain specificity. Whether this finding has any clinical relevance in regard to 
      immunoprophylaxis remains to be determined.
CI  - Copyright 2002 Elsevier Science B.V.
FAU - Mengeling, William L
AU  - Mengeling WL
AD  - Virus and Prion Diseases of Livestock Research Unit, National Animal Disease 
      Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010, USA. 
      bbmengeling@aol.com
FAU - Lager, Kelly M
AU  - Lager KM
FAU - Vorwald, Ann C
AU  - Vorwald AC
FAU - Koehler, Kenneth J
AU  - Koehler KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Body Weight
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - DNA, Viral/chemistry/genetics
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Lung/pathology/virology
MH  - Lymph Nodes/pathology/virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/blood/immunology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/pathogenicity
MH  - Species Specificity
MH  - Specific Pathogen-Free Organisms
MH  - Swine/*immunology
MH  - Vaccination/methods/*veterinary
MH  - Vaccines, Attenuated/immunology/standards
MH  - Viral Vaccines/*immunology
MH  - Virulence
EDAT- 2003/02/20 04:00
MHDA- 2003/06/17 05:00
CRDT- 2003/02/20 04:00
PHST- 2003/02/20 04:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2003/02/20 04:00 [entrez]
AID - S0378113502004273 [pii]
AID - 10.1016/s0378-1135(02)00427-3 [doi]
PST - ppublish
SO  - Vet Microbiol. 2003 May 2;93(1):13-24. doi: 10.1016/s0378-1135(02)00427-3.