PMID- 12592615
OWN - NLM
STAT- MEDLINE
DCOM- 20031003
LR  - 20131121
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 38
IP  - 1
DP  - 2003 Jan
TI  - Body temperature and heat production in suckling rat endotoxaemia: beneficial 
      effects of glutamine.
PG  - 37-44; discussion 37-44
AB  - BACKGROUND/PURPOSE: Sepsis is an important cause of neonatal mortality. The aim 
      of the study was to investigate the metabolism of endotoxic neonatal rats and the 
      potential beneficial effect of glutamine. METHODS: Suckling rats received 
      intraperitoneal saline (control; C), endotoxin (300 microg/g LPS; E), 
      saline+glutamine (2 mmol/g; CG), endotoxin+glutamine (EG), saline+leucine (2 
      mmol/g; CL) or endotoxin+leucine (EL). Sepsis score (0-8) and rectal temperature 
      were monitored. Hypothermia was defined as rectal temperature less than 32 
      degrees C. Oxygen consumption (VO2, mL/kg/h), a determinant of heat production, 
      was measured by indirect calorimetry. Data (mean +/- SEM) were compared by 
      analysis of variance (ANOVA), paired t test or Fisher's Exact test. RESULTS: 
      Endotoxic (E) rats had significantly lower VO2 than C rats from 90 minutes 
      postinjection to the end of the experiment, 210 minutes (VO2 from 150 to 210 
      minutes: C 671 +/- 45; E 429 +/- 36, P <.0004; n = 8; paired t test). VO2 of CL 
      or CG rats was elevated between 90 and 210 minutes compared with control, but 
      significantly (P <.01) only in the L group (C 706 +/- 31; CG 871 +/- 63; CL 984 
      +/- 31; n = 7-9, ANOVA). VO2 was significantly higher (P <.05) in EG rats than E 
      rats (E 460 +/- 29; EG 654 +/- 68; n = 9-10). In the EL group, VO2 was raised but 
      was not significantly different from E (E 460 +/- 29; EL 637 +/- 52; n = 8-10). 
      EG rats were significantly less hypothermic between 90 and 210 minutes (58 of 132 
      measurements) compared with E (95 of 147; P =.0007, Fisher's Exact test), whereas 
      the EL group were similarly hypothermic (74 of 120) to E (P =.7). Sepsis score 
      was significantly lower in the EG group than both E and EL groups (E 4.9 +/- 0.3; 
      EG 3.6 +/- 0.3; EL 5.0 +/- 0.3; n = 40; P <.01; ANOVA). CONCLUSIONS: Neonatal 
      endotoxaemia lowers VO2, heat production, and body temperature. Glutamine and 
      leucine both cause nutrient-induced thermogenesis in control animals and restore 
      VO2 of endotoxic animals. Glutamine additionally increases rectal temperature, 
      reduces incidence of hypothermia, and improves clinical signs of endotoxic rats. 
      This suggests that glutamine may be beneficial for nutrition in neonatal sepsis.
CI  - Copyright 2003, Elsevier Science (USA). All rights reserved.
FAU - Garrett-Cox, Robin G
AU  - Garrett-Cox RG
AD  - Department of Paediatric Surgery, Institute of Child Health, London, England.
FAU - Pierro, Agostino
AU  - Pierro A
FAU - Spitz, Lewis
AU  - Spitz L
FAU - Eaton, Simon
AU  - Eaton S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0RH81L854J (Glutamine)
RN  - 142M471B3J (Carbon Dioxide)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Animals, Suckling
MH  - Basal Metabolism/drug effects/physiology
MH  - Body Temperature/*drug effects/physiology
MH  - Carbon Dioxide/metabolism
MH  - Endotoxemia/*drug therapy/metabolism/physiopathology
MH  - Energy Intake/drug effects/physiology
MH  - Energy Metabolism/drug effects/physiology
MH  - Glutamine/pharmacology/*therapeutic use
MH  - Leucine/pharmacology/therapeutic use
MH  - Oxygen Consumption/drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Rectum/drug effects/physiology
MH  - Thermogenesis/*drug effects/physiology
EDAT- 2003/02/20 04:00
MHDA- 2003/10/04 05:00
CRDT- 2003/02/20 04:00
PHST- 2003/02/20 04:00 [pubmed]
PHST- 2003/10/04 05:00 [medline]
PHST- 2003/02/20 04:00 [entrez]
AID - S0022346802630143 [pii]
AID - 10.1053/jpsu.2003.50006 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2003 Jan;38(1):37-44; discussion 37-44. doi: 
      10.1053/jpsu.2003.50006.