PMID- 12594253
OWN - NLM
STAT- MEDLINE
DCOM- 20030521
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 170
IP  - 5
DP  - 2003 Mar 1
TI  - Induction of low dose oral tolerance in monocyte chemoattractant protein-1- and 
      CCR2-deficient mice.
PG  - 2316-22
AB  - The chemokine monocyte chemoattractant protein-1 (MCP-1) and its receptor CCR2 
      have been shown to play an important role in the migration and trafficking of 
      macrophages and Th1 effector cells in experimental autoimmune encephalomyelitis. 
      Also, MCP-1 has been reported to regulate oral tolerance induction by inhibition 
      of Th1 cell-related cytokines and by the ability of Abs to MCP-1 to inhibit oral 
      tolerance. This study demonstrates that neither MCP-1 nor its receptor CCR2 is 
      required for the induction of oral tolerance. Mice deletional for either MCP-1 or 
      CCR2 had suppressed cell-proliferative and Th1 responses following oral 
      administration and immunization with myelin oligodendrocyte glycoprotein 
      (MOG(35-55)). TGF-beta was up-regulated in fed and immunized deletional mice, 
      while IL-4 was absent from deletional mice, but up-regulated in controls. 
      Decreased experimental autoimmune encephalomyelitis severity was found in 
      MOG(35-55)-fed MCP-1 deletional mice, indicating induction of oral tolerance. 
      These results demonstrate that MCP-1 is not required for induction of oral 
      tolerance and that MCP-1 and CCR2 are essential for up-regulation of IL-4 in 
      tolerized mice.
FAU - Gonnella, Patricia A
AU  - Gonnella PA
AD  - Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical 
      School, Boston, MA 02115, USA. patricia.gonnella@tch.harvard.edu
FAU - Kodali, Dhatri
AU  - Kodali D
FAU - Weiner, Howard L
AU  - Weiner HL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycoproteins)
RN  - 0 (Myelin-Oligodendrocyte Glycoprotein)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (myelin oligodendrocyte glycoprotein (35-55))
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*deficiency/*genetics/metabolism
MH  - Dose-Response Relationship, Immunologic
MH  - Encephalomyelitis, Autoimmune, Experimental/genetics/immunology/pathology
MH  - Female
MH  - Glycoproteins/administration & dosage/immunology
MH  - Immune Tolerance/*genetics
MH  - Immunohistochemistry
MH  - Injections, Subcutaneous
MH  - Interleukin-4/biosynthesis
MH  - Intestinal Mucosa/chemistry/immunology/metabolism
MH  - Intubation, Gastrointestinal
MH  - Lymphoid Tissue/chemistry/immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myelin-Oligodendrocyte Glycoprotein
MH  - Peptide Fragments/administration & dosage/immunology
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*deficiency/*genetics
MH  - Up-Regulation/genetics/immunology
EDAT- 2003/02/21 04:00
MHDA- 2003/05/22 05:00
CRDT- 2003/02/21 04:00
PHST- 2003/02/21 04:00 [pubmed]
PHST- 2003/05/22 05:00 [medline]
PHST- 2003/02/21 04:00 [entrez]
AID - 10.4049/jimmunol.170.5.2316 [doi]
PST - ppublish
SO  - J Immunol. 2003 Mar 1;170(5):2316-22. doi: 10.4049/jimmunol.170.5.2316.