PMID- 12595089
OWN - NLM
STAT- MEDLINE
DCOM- 20030708
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1620
IP  - 1-3
DP  - 2003 Mar 17
TI  - Inhibition of formyl-methionyl-leucyl-phenylalanine-stimulated phospholipase D 
      activation in rat neutrophils by the synthetic isoquinoline DMDI.
PG  - 191-8
AB  - The expression of phospholipase D (PLD) isoenzymes in neutrophils was 
      investigated using reverse transcription-polymerase chain reaction analysis. 
      Amplification products of predicted size were obtained from rat neutrophils with 
      nucleotide sequences corresponding to PLD1a and PLD2. 
      1-(3',4'-Dimethoxybenzyl)-6,7-dichloroisoquinoline (DMDI) inhibited the 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PLD activation in rat 
      neutrophils. The underlying cellular signaling mechanism of DMDI inhibition was 
      investigated. The fMLP-induced protein tyrosine phosphorylation and the membrane 
      translocation of ADP-ribosylation factor (ARF) and Rho A in neutrophils was 
      attenuated by DMDI in a concentration-dependent manner. However, neither the 
      membrane association of protein kinase C-alpha and -beta isoenzymes in 
      fMLP-stimulated cells nor the GTPgammaS- and phorbol 12-myristate 
      13-acetate-stimulated membrane translocation of ARF and Rho A in a cell-free 
      system was affected significantly by DMDI. These results indicate that the 
      expression of PLD1a and PLD2 mRNA in neutrophils. Attenuation of protein tyrosine 
      phosphorylation and the membrane association of ARF and Rho A probably play a 
      concerted role in the inhibition of PLD by DMDI in rat neutrophils in response to 
      fMLP.
FAU - Chang, Ling-Chu
AU  - Chang LC
AD  - Department of Education and Research, Taichung Veterans General Hospital, 160, 
      Chung Kang Road, Sec. 3, Taiwan, ROC.
FAU - Chen, Chi-Ming
AU  - Chen CM
FAU - Wang, Jih-Pyang
AU  - Wang JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (1-(3',4'-dimethoxybenzyl)-6,7-dichloroisoquinoline)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoenzymes)
RN  - 0 (Isoquinolines)
RN  - 0 (RNA, Messenger)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factor 1)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - ADP-Ribosylation Factor 1/metabolism
MH  - Animals
MH  - Cell Membrane/drug effects/enzymology
MH  - Cell-Free System
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Isoenzymes/antagonists & inhibitors/metabolism
MH  - Isoquinolines/*pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Neutrophils/drug effects/*enzymology
MH  - Phospholipase D/*antagonists & inhibitors/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Kinase C/metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - rhoA GTP-Binding Protein/metabolism
EDAT- 2003/02/22 04:00
MHDA- 2003/07/09 05:00
CRDT- 2003/02/22 04:00
PHST- 2003/02/22 04:00 [pubmed]
PHST- 2003/07/09 05:00 [medline]
PHST- 2003/02/22 04:00 [entrez]
AID - S0304416502005329 [pii]
AID - 10.1016/s0304-4165(02)00532-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2003 Mar 17;1620(1-3):191-8. doi: 
      10.1016/s0304-4165(02)00532-9.